Rat Dialysis Device Animations

This post is not an update on my research. I am creating this page to house download links to the COMSOL Multiphysics models I’ve constructed in the course of my work here in the McGrath Lab. This page is linked to in my thesis for legacy purposes. Important: these files have been uploaded with the…

In the previous post, I discussed how we could evaluate the flux of molecules through the membrane and alluded that this method could be used similarly to our effective diffusion coefficient manner of evaluating membrane impact.  Here are results of the full comparison. Standard setup: 15nm membrane, 1 hour time point, 1mm fluid wells, 5%…

Introduction As a continuation to my previous post, I am running a triplicate of cell culture adhesion tests to observe cell lose on our membranes following physiological shear stress. Methods HUVECs (P3,4,5) were seed within our mimetic and left to grow to confluent for 24 hours. Shear (4.5 dyn/cm2) was initiated and images were collected…

I performed separations using C-dots from Hybrid Silica, a silica based particle with an internal fluorescent core.  The particles are coated with a PEG layer of unknown length, but the idea would be that they’d be less affected by electrostatics than gold.  I used two sizes.  One is marked <10 the other <30, but I…

Earlier in the week I retested the 100K microcons in our wet-wet EQ format. When I last ran them this way I calculated very little flow and very small permeability values. I noted that there was a rather large air pocket collecting on the underside of the membrane in the plastic support structure; most likely…

SU-8 3010 has been used to create a scaffold in the fabrication of the liftoff membranes. The supplier of the negative resist, MicroChem, rates it for a 13 moth shelf life. We were having difficulty achieving good liftoff with membranes in the second half of last year. The SU-8 had expired by several months, so…