Updated Burst Pressures Picture

The following post is the abstract that I recently submitted for the BMES Annual Conference. Introduction: Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and…

I’ve been thinking a lot about energy storage lately, and I think I’ve found a potential use for pnc-Si in Supercapacitors. What is a supercapacitor? As the name suggests, it’s a double-layer capacitor (EDLC) with very high capacitance values (1-500+ Farads) or a million times more than that of a typical dielectic capacitor. An electrolyte…

Samples are from wafers #316 and 318. This is the first time for wafer scale deposition. Processing conditions:1) chamber base pressure @ 6×10-7 Torr (previously @ 5×10-6 Torr)2) 15nm Ti + 15nm Au, no vacuum break Estimated “cell resistance”: about 20-25 ohm, much lower than previous measurement. Possible reasons why lower resistance is observed:1) “Au…

Previously I have shown that sample buffer can be used to lift adsorbed proteins off polymer membranes, which can then be imaged on a gel. I have used a similar procedure to assay adsorption on pnc-Si membranes. I used 4 samples from wafer 216 that all had pinholes. I incubated a drop of 1mg/mL BSA…

We’ve been wondering about the effects of membrane stresses on discoloration rates.  This was spurred by Vlassak’s visit and his ideas about stress and membrane stability. In this experiment, I used w501, no RTP, in the oven in RPMI+10%FBS.  All of these samples were in the Sepcon format: chip sealed with a fat O-ring, chip…

We used chips from the latest 5 slot SepCon NPN wafer 1142 (14%, 36nm) to test our ability to separate 20 nm  “blue  fluorescent” polystyrene NPs from Invitrogen from IgG. We used a concentration of 10^14 particles/ml NPs and 1 mg/ml IgG.  (This is a mixture of fluorescent and unlabeled IgG) We compared forward vs…