Discoloration presentation

We’ve been moving to a new format for chips, trying to standardize an active area that has good yield and allows fluidic access through the chip itself. The SEPCON outer dimensions have been reused (5.4 mm x 5.4 mm). It’s a 1.5 mm^2 single slot chip area (2 mm x 0.7 mm) with vias that…

This is more work that Randi did looking at ADSCs migrating towards HUVEC networks.   Motivation: To demonstrate the migration of ADSC toward pre-formed HUVEC networks when ADSC are seeded 4 hours after HUVEC. Method: Monday – Coat 4 wells of a 96 well plate with 100% Geltrex, place in incubator. Seed 20,000 HUVEC cells in…

This will be a very brief post, but kind of an interesting one. I have been attempting to get my identification of exosomes by immunogold labeling and that hasn’t been going terribly well. I think that it is due to a wealth of complications including a lack of fixed exosomes, salt contamination and other problems….

Our main purpose of this project is to reduce or eliminate the protein non-specific adsorption on the oxidized siliconnitride sample surface. By synthesis the PEG molecular on the surface, we believe we can reach the goal. There are different ways to synthesis the PEG on the surface. First is the silanes, after synthesis the silanes,…

When we are trying to optimize the capture of exosomes in the tangential flow system, it is important to understand how particles are behaving in the flow. Initially, we tried to do this last summer by looking into the particle tracing package for COMSOL, but that required a complete overhaul of the software (a new…

Last week’s production yielded two wafers (502 & 504) with defect-free membranes. 506 had a high pinhole density. These wafers have a TEOS mask and were annealed twice; the first anneals did not produce pores so they were annealed again at 1000 C. From these results, it seems that the first anneal “seeds” pore formation…