Discoloration presentation

As mentioned previously, gold colloids are not stable in high salts because salts reduce the repulsive forces between particles and cause aggregation.  I have been able to dissolve naked gold colloids in 10mM KCl without aggregation, but the particles crash out at 100mM KCl.  There are a couple of strategies that can be used to…

Last week, I looked at discoloration with different wafer numbers (639,670,672,683,685,702,703), pre- and post-rebuild.  This experiment was done in the oven at 37C, in PBS and without RTP.   Based on the starting color, w670 and w672 are the thinnest membranes.  These discolored rather quickly (1 day).  The only other membrane that discolored over 3…

Introduction This post is meant to introduce the new NPN-enabled slide-based devices produced by Ibidi. These devices feature an NPN membrane and microfluidic channels positioned perpendicular to one another formed by PSA and COP layers (Figure 1). Channels above and below the membrane allow for flow-based experiments including tangential flow filtration (TFF) such that biofluids…

The aim of this work was to electrospin aligned polymeric fibers on parylene stencils as means to characterize the critical necessary total area and necessary opening sizes to elicit endothelial cell response in the form of cell alignment among other characteristics typically found in human cells that are adjacent to highly aligned ECM. For this…

In these discoloration tests, I looked at the components of HBSS.  HBSS consists of NaCl, KCl, 0.048 mg/mL Na2HPO4, 0.06 mg/mL KH2PO4, glucose and 0.35 mg/mL NaHCO3.  In a previous post, I showed that NaCl, KCl and glucose didn’t cause discoloration (previous post).  So, I made HBSS from scratch and I combined the various phosphate…

Xingyu et al; Supplement The authors of this submission to ACS Nano apply techniques for the creation of mesoporous silica thin films with vertically aligned nanochannels to create ultrathin (< 100 nm) silica membranes with ~ 2 nm nanochannels. The technique involves the growth of silica around surfactant templates followed by the removal of the…