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Fluidics and cultured cells on pnc-Si
As a proof-of-principle, Henry and I have been working on a microfluidic device for flowing blood cells/particles across cultured cells on pnc-Si. More on why this is important later. We’ve come up with a prototype and I’ve been testing it out. I will include schematics later but here are the first couple of movies I’ve…
NSL fabrication – Released membrane pictures
This post includes some optical and SEM micrographs of a SiO2 membrane patterned by nansosphere lithography using 300 nm polystyrene beads. An SU-8 microgrid was deposited on top and the membrane was released by dissolving a bottom layer of ZnO. After the release, the membrane is rinsed in water and transferred to a clean substrate….
Auger Electron Spectroscopy: A Basic Overview
ByKileanSince I am posting data that is acquired using Auger electron spectroscopy, I thought that it might be a good idea to write a post about the theory of the instrument, to better understand why this data is useful and relevant. The Auger effect is a phenomenon in which an atom that is excited by…
The Completion of the First Stage of the Gauntlet (Knocking Aim 1 Out of the Park)
ByKileanThis post has been a long time coming, but I have finally (essentially) finished all the work for Aim 1 of my thesis. (Credit to Dan for helping with part of it and getting a thesis along the way, see his post here: https://trace-bmps.org/critical-flux-of-ultrathin-silicon-membranes-in-tangential-flow-filtration-of-protein-solutions/) Up until this point, I have been attempting to validate my…
Beads experiments with NPN membranes
ByAslanHello Everyone, For the next figure in our paper, and to make the transition happens smoothly from Track Etched membranes to NPN membranes, we decided to run the same beads experiment on NPN membranes as well. So Kilean and i started working on these experiments, and the first experiment was to capture and release 100…
Rotation Project Complete: Dialysis of cytochrome c across a commercial membrane
Dan Steiner Rotation Summary Abstract The purpose of this rotation project was to develop and test a clampable system for eventual use in scalable nanomembrane dialysis systems. The nanomembrane dialysis system will be miniaturized into a compact, wearable hemodialysis device that will provide more effective human dialysis treatment (Johnson et al. 2013). For initial tests, a…