Nanoporous tent wetting fluorescence inconclusive

15um beads on discolored membrane surface, having some slits where the beads are not found The localization of microbeads suggest that they have fallen across the slits in the membrane Transparent membrane with microbeads on its surface These membranes are not treated with cell media, hence are not discolored. There’s a slit which appears to…

For context, see my previous post. Early on in the process of designing an experiment to confirm the usefulness of a CytoVu assembly for use as an assay, it was decided that the best solution for measuring the extremely low concentrations [ng/mL scale] of bacterial alkaline phosphatase (“bAP”) in the filtrate which would indicate what…

Tweaking simulation was not bringing it any closer to the experimental shown in the last post.  This led me to try the experimental once more.  I set up the same system and used 1:2 dilution of the standard protein mixture.  I then homogenized the retentate and filtrate and ran 10 ul of each on a…

(This post is not finished, but my flight keeps getting delayed in 20 minute increments, so better to publish it unfinished than to not get it up before NRG in case I’m not able to make it. Please forgive typos etc.) I attended a meeting organized by the Kidney Health Initiative, a collaboration between the…

These are a couple of figures that may appear in the supplementary materials for the permeability paper. The first tries to explain that most of our permeability is due to the largest pores.  The lines plotted are the cumulative number of pores at each size (blue) and the cumulative permeability (green). The lines show that…