Nanoporous tent wetting fluorescence inconclusive

I recently popped out membranes from wafers 1064 and 1065 for our lab. I only started with about half a wafer, but these are the numbers from the extraction: Wafer ID # of In-Tact Extracted Membranes : High/Low Porosity # I broke during extraction # already broken before extraction # apparently under etched 1065 (100W)…

The following post is the abstract that I recently submitted for the BMES Annual Conference. Introduction: Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and…

In these discoloration tests, I looked at the components of HBSS.  HBSS consists of NaCl, KCl, 0.048 mg/mL Na2HPO4, 0.06 mg/mL KH2PO4, glucose and 0.35 mg/mL NaHCO3.  In a previous post, I showed that NaCl, KCl and glucose didn’t cause discoloration (previous post).  So, I made HBSS from scratch and I combined the various phosphate…

Our previous work with reverse centrifugation NPN membranes has shown that they may work for cleaning up excess protein from conjugated NPs.  This initial success used ~20 nm red fluospheres with membranes from wafer 1070 (10-15%, ~45nm) and unlabeled IgG we were given by a collaborator.  In this post we are testing “newer” NPN wafers…

We’ve built a set up for this but it’s not incredibly user friendly right now.  It consists of a camera and a stage.  Using the macro function of the camera you can take a close up image of the chip, but you must make sure the horizon line is straight i.e. camera and stage are…

In my last post I showed that using buffered solutions instead of Di-water we could obtained similar results with SC256 as we had with SC269. I used 20mM HEPES at pH7.5 with no salts and showed DDM diffusion through both “treated” and “untreated” SC256 samples. Additionally I showed that “treated” SC256 samples appeared to slow…