Nanoporous tent wetting fluorescence inconclusive
Due to blog troubles yesterday, we have chosen to upload this post as a .pdf: Nanoporoustentwettingfluorescenceinconclusive
Due to blog troubles yesterday, we have chosen to upload this post as a .pdf: Nanoporoustentwettingfluorescenceinconclusive
AFM images report Protocol: pnc-Si TEM grids were mounted on thin silicone inserts. Surface treatments were applied to the grids. Except for the CellTak treatment, the treatments were all diluted in Hank’s Balanced Salt Solution (HBSS) and incubated with grids for 2 hours and then washed off with PBS. CellTak was diluted in 0.1 M…
I mixed made solutions of PBS and Rhodamine with varying Rhodamine concentrations and ran them through the spectrometer. Below is the concentration vs absorption curve I made from it. I blanked the spec with PBS instead of ddH2O since the solution is in PBS. The slope is almost double for Rhodamine in ddH2O compared to…
As Jim suggested, we are not only going to reproduce what Chris and Tom did in the Nature paper but also trying to test the charge screening effect based on different salt concentrations. To do such experiments, we need to charge our Si membrane and at the same time oxidize it. At the end of…
This post is related to the HIV drug screening project that Carrie has been working on. To refresh everyone’s memory, the aim of this project is to use pnc-Si as a filter to separate high molecular mass (HMM) protein-RNA complexes from smaller protein monomers/multimers. Small molecules that break down the HMM form of this protein…
Paper: Oo2016 Supplementary Info:Oo2016SI Abstract: Design and fabrication of three-dimensionally structured, gold membranes containing hexagonally close-packed microcavities with nanopores in the base, are described. Our aim is to create a nanoporous structure with localized enhancement of the fluorescence or Raman scattering at, and in the nanopore when excited with light of approximately 600 nm, with a view…
Since we’ve been noticing slower discoloration in the Sepcon format, I looked at the 2 components of Sepcons – the plastic and the O-ring. Specifically, I cut pieces from w622 and incubated them in FBS-DMEM/F12 either alone, with the Sepcon plastic or with an O-ring. This was in the oven, and the chips were membrane…
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There is a method for direct determination of mass within the optical path which may be valuable here, and produces interference fringes similar to what i can already see in your images:
http://en.wikipedia.org/wiki/Classical_interference_microscopy
When Greg is in Nottingham later in the year we can make a pilgrimage to a colleagues scope in Glasgow and quantify this directly, if you haven’t cracked it already by then 🙂