We’ve built a set up for this but it’s not incredibly user friendly right now. It consists of a camera and a stage. Using the macro function of the camera you can take a close up image of the chip, but you must make sure the horizon line is straight i.e. camera and stage are in same plane.
The angle function in ImageJ can be used to find the contact angles.
Summary This post complements Fabricating 0.5 um Channels in Microscope Slides for Staph Invasion Assays. Challenges in the etch transfer process necessitated switching to a different substrate that had stronger selectivity. With that in mind, we set out to create 500×500 nm channels in silicon wafers for the bone invasion study. We had partial success (micron-sized…
Hi all, For the past one month I have been working on a height-based device for culturing bEnd3 (mouse brain endothelial cells) under flow. Ideally, this device is to constitute a blood brain barrier (BBB) mimetic. Device Full View (Front): The fluid flow is driven by hydrostatic pressure, created through the differences in the…
Previous work on Tomograms: TEM Tomography of NPN and npMgF2 Previous work on reconstructing the tomograms: Manual Reconstruction of Electron Tomograms Introduction This post examines another set of MgF2 pores that Kilean acquired. I want to compare the inner pore morphology of two different processing flows for MgF2 nanomembranes, one of which gives us excellent SERS properties (250C…
We are trying to reproduce the very small pores Joe created with 30/100/30 OSO and large pores created with 30/100/30 NSN using a SEPCON pattern. (Joe’s previous post.) We had a problem with several chips releasing from the center of the wafer prior to completely clearing all the features. In the future we will use…
In evaluating the usefulness of our membranes for cell culture, it is important to understand if and how cell growth is affected by the surface. The desire is that they be consistent with standard culture substrates (i.e. Tissue Culture Plastic (TC)). I performed two growth trials using HUVECs (Human Umbilical Vein Endothelial Cells), one focusing…
In order to resolve the silicon nitride- silicone gasket bonding issue, I, Josh, Tucker and Greg used different approaches to functionalize the nanoporous nitride (NPN) surface. Both surfaces of NPN, although have same material (silicon nitride), behave differently. The flat side is etched using xenon fluoride to remove pncSi layer on top. Different tools at…
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Droplet looks fuzzy. Maybe that is just fine?
This will likely be sensitive to to drop volume and whether there was any lateral movement as the drop was applied. Have you found it to be repeatable, or have you implemented a detailed procedure to minimize these effects?
It’s not the perfect system yet for controlling the drop. This was done with a micropipette, so drop volume is repeatable, but not position. We can try to rig a stand or something for this.
If you find that the measurements are not consistent, then a simple stand might help. It may not be necessary, though. Trying to drag the spot around and making advance/retreat measurements may also help..