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Incorporating a ECM hydrogel into an in vitro microvasculature system
ByLeslieFor the past month, I have been working along with Tejas towards incorporating an ECM-like hydrogel into the back (edged side) of a pnc-Si chip. The purpose of this effort is to add another layer of complexity to Tejas’ in vitro microvasculature system, in order to study neutrophil migration across the endothelium. We first pondered…
Review paper: Fabrication Techniques Enabling Ultrathin Nanostructured Membranes for Separations
As you may already know, we are writing a review article for the Electrophoresis journal on nanofabrication methods. This post is to request your feedback on the figures included in the manuscript. The main objective of figure 1 is to compare top-down and bottom-up processes to justify the use of the methods reviewed in the…
Rhodamine and Fluoroscein Diffuse through SC269
ByMike HYesterday I posted my results from a BSA diffusion experiment using “treated” and “untreated” SC269 samples. To ensure that the pores in SC269 are indeed open I took a step backwards and set up a diffusion experiment using small dye molecues; rhodamine and fluoroscein. Using Jess’ diffusion cell I set up a matrix to test…
Double anneal
This is a follow up to Jess’ protein separation post. She mentioned that some of the SC 073 samples were annealed twice: the first was a 700 C 60 s anneal to crystallize (before etching) and the second was a 800 C 5 min anneal to stabilize the membrane (after etching). I took some TEMs…
UV/Ozone cleaner operation reminder
ByJimMaryna told me the UV/ozone cleaner did not work (the blue light in front of the chamber did not light up) when she was working with Dave today. I quickly checked the machine and it is actually working properly. The trick is to make sure you trigger the “safety lock” behind the chamber before you…
