Paper review- A versatile lab-on-a-chip tool for modeling biological barriers
This paper parallels my efforts in creating a microfluidic device that can perform co-culturing of different cell types, allow real time phase and fluorescence imaging, and perform non-invasive TEER measurement using transparent electrodes. This is almost exactly what we aimed for; the only difference is in the type of the membrane. They are using a 23 micron very low-porosity polymer membrane. Low-porosity (2×10^6 pores/ cm2, pore dia = 0.45 micron) is significantly low to our material, which also allows them to take great images in phase. Nonetheless, the paper establishes co-culturing data for gut, lung, and brain cells, and in fact, grows brain ECs under shear flow all while recording TEER data.
The membrane website:http://www.it4ip.be/en_US/products/cell-culture.html
The suppliers in USA: http://www.arbrown-us.com/newproducts.html
This is the original paper:
walter_BBB_supplementary data Supplementary data
Walter_BBB_chip_SensorsActuatorsB_in_press Actual paper
The presentation and review: [15-10-06] Journal Club_cucullo visit