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Track-Etched Membrane Update
ByMike HThis morning I did a little work with the track etched membranes (0.05um 13mm). I talked with Paul over in Hopeman and he is currently working on making a small coring tool to cut out the membranes (it should be done by the end of the day). In the mean time I figured out that…
MgF2 Cytocompatibility #4
Summary: I have some ok images (20x) of cells growing on MgF2. My controls still look poor. Something about the stain is causing the TCP + NPN controls to detach ahead of the MgF2 material, even if they aren’t fully dead yet. I used acetone fixation, which worked well for the materials on the nanomembrane, but…
Fisher @ NRG, Summer 2017
ByFernandoWe are happy to be back this summer. Anthony has been working on setting up a system that mimics Kilean’s dialysis devices. Our approach is different in two important ways. First, we are using PETLs, instead of PDMS/glass. For those who are not familiar with PETLs, here is a short summary: Commercial laminating pouches made…
Contact area dictates early cell-substrate interaction for endothelial cells on porous membranes
ByAnaAbstract Endothelial cell attachment to porous substrates via focal adhesions is initially affected by pore spacing and not absolute porosity. Maturation of endothelial monolayers via cell-cell interactions occurred over the same time course regardless of substrate porosity, pore size and contact area. Cell-matrix interactions at maturity were dictated by substrate stiffness. Data [missing figure] Fig. 4 Endothelial…
Exosome Paper Figures
ByAslanFigure 2 – Micro scale Experiments with 10 micron fluorescent particles and 8 micron pore size track etched membranes: A) Fluorescent intensity – time plot showing increasing in the intensity signal during the capturing step and decreasing for releasing step. B,C and D) Before capturing, After capturing and After releasing panels, respectively, including schematic images…
NSF/GOALI supplemental award: Bringing Nano to Undergraduates @ Fisher
ByFernandoHello NRG! Thanks to Jim McGrath and NSF, St. John Fisher College students and myself will have the opportunity to conduct research at URNano and the McGrath Lab this summer. I am an Assistant Professor at Fisher in the Biology Department, and my background is in cellular immunology, with a focus on sterile inflammation. Why…
