Gold sieving graph with all my data and model lines
Similar Posts
n-Dodecyl β-D-Maltoside Coating on PDMS
Today I performed a coating of n-Dodecyl β-D-Maltoside (“DDM”) on PDMS in an attempt to block protein adsorption (reference). The reference describes two different procedures, one in which the protein being incubated on the PDMS surface is in solution with DDM, and one in which the DDM coating is performed before the sample is washed…
Endothelial Focal Adhesion BMES Abstract 2015
The following post is the abstract that I recently submitted for the BMES Annual Conference. Introduction: Permeable membranes provide a physiologically relevant cell culture substrate for in vitro study of barrier cell types, such as endothelial cells, which assume a polarized morphology with distinct apical and basolateral surfaces. Cell morphology and subsequent monolayer formation and…
Desalting: Intact SepCons
Introduction One area of use to explore for the membranes is desalting, the removal of salts from solutions (such as those containing proteins). Ideal membranes have uniform pore distributions, high mechanical strength, tunable, sharp pore cutoffs, and high porosity. In this study intact samples from W565, which showed no air permeability but allowed rhodamine to…
Adhesion Testing: HUVEC and Fibroblasts
Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities are due to the cells rolling of the edge of the raised pnc-Si substrate or…
HUVEC Spreading on Various Substrates
To continue our spreading and proliferation experiments on various topographical features and stiffness, I repeated the HUVEC experiments I had done before due to a possible artificial bias that we think is a result of high seeding. To enusre accuracy, I repeated the experiment and added another plate to test the HUVECs on substrates of…
Rhodamine 6G diffusion through RTP treated and non-treated pncSi (w/o pinholes)
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no significant difference in diffusion between the samples after they are RTPed, as expected. Now, Henry…