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TECAN absorbance for 96-well plates is topping out even with pure buffer
EDIT: This problem was solved thanks to Tejas. It turns out that Falcon BD transparent 96 well plates absorb in the UV range, and are best used for bradford assays or anything that measures absorbance higher than the UV range. Apparently they sell UV-inactive 96 well plates, but they are very expensive. Josh Winans and…
Diffusion Stir Cell Update
Mike and I tested the movement of Rhodamine between the two cell channels to prove that the Rhodamine particles do diffuse through the membrane. After inspection in the microscope we saw that the fluorescence from the Rhodamine does transfer from one channel to the other across the membrane. However, we also noticed that the Rhodamine…
The Long Road to Wearable Dialysis
Directory containing article and presentation Nature Article about Willem Kolff
Depleted Serum Separation Attempt
I’m currently trying to compare separations between different complex protein mixture. This was my first crack at depleted human serum. This mixture has been processed so that the most common blood proteins (such as albumin and immunoglobulins) have been removed. All three trials were performed using membranes from w338 in diffusion mode. 338 has a…
Secrets of the NSF
ByJimWhile serving on an NSF MRI (Major Research Instrumentation program) review panel today the director shared his analysis of funding probabilities as a function of score. The analysis was only done for the Directorate of Math and Physical Sciences, but I bet it is a good rule of thumb throughout NSF. I sketched his power…
peg X-linking
Purpose: The purpose of this investigation was to study the diffusion of Rhodamine, a florescent dye, in crosslinked Poly ethylene glycol (peg) using a Leica DMI 6000. Knowing the permeability of crosslinked peg is valuable as peg is a potential material for the hemodialysis project particularly the lift off component, and knowing about Rhodamine’s (MW…