ICNMM Presentation

This is the first set of studies to assess the hemocompatibility of pncSi surfaces based on the paper by Fissell and Roy. It looks for the behavior of platelets in the plasma upon interaction with pncSi surfaces. Biological Motivation: Platelets are one of the most important components of the human immune system and also one…

The basic principle of inducing the osmotic pressure to suppress back pressure is based on the counterbalance of the opposing flows of solvent.  Consider an electrolytic cell composed of a pair of Ag/AgCl electrodes and a Nafion-coated pnc-Si membrane separating NaCl solution into two halves. Initially, the electrolyte in either half has the same concentration…

Since my last update things have been going quite smoothly. Most pores I make are able to pass 3 or 4 samples before clogging, and I have filled in the various graphs quite nicely. I still would like to flesh out the low and high end of the DNA length spectrum, but I’ve started writing…

I recently completed some experiments with the Endohm system, which measures transendothelial electrical resistance (TEER).  I seeded either 16.25k or 32.5k HUVECs (P5) on commercial polycarbonate (PC) or polyester (PE) transwells and on pnc-Si in the transwell format.  I then tracked the TEER for 7 days.  This data is normalized to the day 0 TEER…

I used the following parameters in the Simple Sieving Model (pardon the MATLAB syntax) Am = 1; %cross sectional area in cm^2 Do = 1*10^-7;% diffusion coefficient in the membrane in cm^2/sec P = .15;% membrane porosity; t = linspace(0,420,500); %time vector sec Qo = 1*0.00166; %militers per second (0.00166 = 10 ul/min) L =…

We’ve been getting some strange cell morphologies recently in short-term adhesion studies.  My previous post on this subject links to a few of these results. Unfortunately, my last experiment into short-term cell morphologies on autoclaved and EtOH- sterilized samples wasn’t well-controlled since I didn’t have enough samples.  Therefore, I repeated this adhesion study with proper…