The Long Road to Wearable Dialysis

To image the blood side (in-channel) side of the membranes, I transferred the membranes of a HD chip onto another wafer segment (SiN wafer: 4534) The transfer wasn’t clean but I got some good images. These membranes were exposed to blood flow for 4 hours in a typical rat HD setup. Post experiment, the membranes…

In this post, I sum up all the NSN stacks with different Si thicknesses I have made so far. The nitride layer is 30nm thick for both the top and the bottom. The thickness for silicon film ranges from 7nm to 10nm, 15nm, 25nm, 50nm and 100nm. All the wafers were firstly etched and then…

Those with a great memory will remember that I’ve been struggling (1, 2, 3) to identify adherens and tight junction proteins (i.e., cadherins and ZO-1) in bEnd3 endothelial cells.  Since I’ve recently moved over to investigate air-blood barrier co-culture models, I decided to see if my epithelial cells (RLE-6TN) express cadherins (they should since these…

Hello Everyone, Please note that Figures are all shown in the powerpoint file. The goal of these experiments was to plot a standard curve for concentration and intensity in my Track Etched Membranes. In order to that, we decided to dry droplets on the membranes with different dilution, then check the fluorescent intensity of them…

For many months, Emma and I are trying to achieve neutrophil migration through endothelial cells and through the underlying collagen gel. I need to explain the details of my device first. The schematic is shown as below. I am using a 3 um porous membrane to allow neutrophils to cross the HUVECs in the collagen…