The Long Road to Wearable Dialysis

I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells.  Here’s a post of images.  I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve been investigating the optimal arrangement of the different cell types (bend3 on top or bottom?). …

I performed separations using C-dots from Hybrid Silica, a silica based particle with an internal fluorescent core.  The particles are coated with a PEG layer of unknown length, but the idea would be that they’d be less affected by electrostatics than gold.  I used two sizes.  One is marked <10 the other <30, but I…

Hi all,   I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius. Best Comittee Meeting

Power Point for my Senior Design   https://docs.google.com/file/d/0B1AH05-mf_tQLTdSMHRWX0NjNGc/edit?usp=sharing  

All of my recent posts on VEGF, VEGFR2 and NF-H imunofluorescence have included data from cells grown on coverslips only.  This was my first attempt to try to do immunofluorescence staining on intact pnc-Si transwells and then pop the chips out of the transwell plastic in order to image at 100X.  Since VEGF is involved…

Background: At the end of August, Greg Schmidt and I were investigating the feasibility of a project that builds off of my PhD research: templating and patterning new materials on top of NPN/microporous nitride. We hope to make composite nanomembranes with various ratios of NPN to sputtered thin films. In addition to my normal TEM,…