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Blood brain barrier 2-color fluorescence
I’ve been trying to get multi-color images of endothelial cells and glial cells on transwell membranes in order to compare image quality on glass, pnc-Si and polymer membranes. Specifically, I’ve tried staining endothelial cells green with VEGF mAbs and glial cells red with neurofulament H (NFH) mAbs but VEGF expression in bEnd3 cells is extremely…
NSN SEPCONs
Still trying to reproduce Joe’s large pore material on SEPCONs using NSN stacks. This post has data from two wafers with 30/50/30 and 30/100/30 NSN. RTP is 1000C 50C/s for 1min. Nitride is etched off by placing samples in 50% HF for 3.5 minutes. (~30s after that the films start to lift off) I start…
Wafer 416-T
Wafer 416-T is a TEOS wafer annealed at 900 C. Pinhole density is relatively low. left: (1,0) @ 35kx; right (0,4) @ 35kx left: (1,0) @ 50kx; right (0,4) @ 50kx
30 nm pnc-Si bias series
We have already established that bias is an important parameter in controlling the morphology of 15 nm thick pnc-Si, but have yet to look at its effect on thicker films. This week, made a few 30 nm thick Si films with 02, 05, 25, and 50 W of substrate bias. I annealed these films at…
Status the 'Harold Smith' Project
ByJimThis is my attempt to summarize where we are with our investigation into the use of membrane arrays in high throughput screening. The effort is known colloquially as the ‘Harold Smith Project’ because the model protein system comes from Harold and is inspired by work in his laboratory on HIV. The principle being tested is…
Single Channel 20 nm membrane breakage…
There is a reliability/handling issue with the single slot chips. 0.5 mm x 10 mm slots, 20 nm thick membranes with 40 nm SiN reinforcement. The membranes are easily packaged in an upper fluidic PDMS layer with inlet/outlets and two PDMS struts (on the membrane side to protect from accidental contact breakage.) The first test…