The role of tRNAs on Cell Phenotypes

For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording.  (reporting a low resistance: 5-10 ohms, shorted). TEER Test…

Cancer cell metastasis is responsible for 80 percent of cancer-related death. The metastatic cells migrate through tight constricting spaces, therefore constricted migration and its enabling mechanisms have received a lot of attentions in recent years. The most famous research groups that are working in this field are Lammerding group, Discher group, Wolf and Friedl group,…

Introduction As a continuation of my rotation project and a beginning to my PhD work in the McGrath lab, I wanted to finalize my shear stress calculations within our blood vessel microfluidic device. All previous shear stress calculations were purely analytical, with many assumptions. This work aimed to minimize those assumptions while providing some publishable…

Two experiments were performed to explore application of SiMPore filters within INT’s microfluidic cartridges to allow the capability for pathogen type specificity in nucleic acid purification. 1) The first experiment tested the efficiency at which nucleic acids could pass through the membranes. T4 polynucleotide kinase was used to 32-P radiolabel two different sized PCR products:…

Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells  and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used as matrices for the cells, the bottom of the plate was used as the control….