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Displaying EO performance
Pnc-Si membranes have a high rate of EO pumping, but what is the best way to compare to other pumps? The initial figure we used is an updated version of a figure found in a paper about alumina EO pumps (original figure bottom of this post). In this figure we show flow rate normalized to…
“Reverse” centrifuge seperations
Back in November we were visited by Tom Berkelman of BioRad to see if he could use our NPN membranes to successfully separate some proprietary nanoparticles from IgG in a solvent with secret ingredients. These particles have an interesting absorbance spectrum as well as strong fluorescence. During that visit and in subsequent separations in the…
Salt diffusion through 'non-porous' SC348
About a month ago, I posted some preliminary data that showed NaCl diffusion through SC348. This was motivated by my observation of vacuole formation on SC348. Since my hypothesis is that cell culture substrate permeability is the key factor for vacuole formation, cells shouldn’t have formed vacuoles on SC348. Unless it is actually porous. This…
Urea/f-BSA/cytochrome c concentrations in retentate after dialysis: 12 hours
Continuing on the path of hemodialysis, we are increasing the complexity of the fluid being dialyzed. In this long-term experiment I will pump 100% serum with added urea (1mM) cytochrome c (1 mg/mL) and fluorescent BSA (1 mg/mL). So I need to detect urea, BSA, and cytochrome c in serum. Cytochrome c absorbs at 405…
HUVEC Adherence on Microporous Membranes Under Flow
ByAlecIntroduction/Motivation Endothelial cell adherence mechanisms are well characterized in literature to date. Endothelial cells, such as HUVECs, interact with their substrate as well as surrounding cells through focal adhesions (network of proteins involved in cell adhesion; vinculin, talin, FAK, etc.), VE-cadherins, tight junctions, and other membrane bound molecules. These molecules work in combination to allow…
Nanoporous SiN Lift-Off Update – April 2015
Background Details: Josh Miller & I have made some recent progress in the NPN LO work. We had several ø6″ wafers w/ 100 nm of thermal oxide processed by Rogue Valley, wherein they deposited 650 nm of poly-Si (LP-CDV) followed by 50 nm of low-stress SiN (LP-CVD). Josh added the a-Si + SiO2 layers at…
