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(Journal Club) Measuring the Zetapotential and Protein Translocation Behavior of Single-pore SiN
This paper (SiN paper) by Ulrich Rant et. Al. details several experiments conducted with a single pore drilled through a SiN membrane via TEM. They measure the relationship of pH vs. zetapotential for both a model protein (avidin) and their membrane, and then see how protein moves across the membrane at different pH’s. Translocation is…
Polymeric Separations
This post outlines two sets of polymer membrane separations using 100kD PES and 30kD cellulose in diffusion mode. The 100kD PES experiment is a repeat of the experiment found here; in the first trial of this work, there was no filtrate remaining on the backside. This time there was filtrate remaining that I could test…
HUVEC migration and Fibronectin Fibrillogenesis
ByHenryIntro: Hi all, as we investigate the formation of HUVEC focal adhesions on porous SiO2 membrane , we also looked at fibronectin fibriollogenesis. Interestingly, it appears that after 24 hr (since the initial seeding), the fibronectin fibrils formed by HUVEC vary depending on the pore characteristics of the SiO2 membrane. In this case, we looked at…
RTP Time Variation for Cell Culture
In an attempt to improve membrane life in cell culture, different RTP times were tried on a single wafer. Samples from W514 were RTPed in Ar at 800C for 1, 5, and 10 minutes, respectively. Cells were then seeded at confluence on membranes, with membrane stability monitored for breakage. Discoloration was monitored on samples without…
Dialysis System Update
ByMike HHenry and I just got back from our meeting with Dean over at RIT to talk with him about the production process for making our proposed flow dialysis system. While we were there Dean described in detail the entire production process including the anodic bonding and the parylene deposition. We also returned with membrane samples…
‘HTTP error’ when trying to upload images
ByJimHello everyone, After some work on GoDaddy’s side, we have fixed the HTTP error when trying to upload images. Please let me know if you encounter this issue in the future. Cheers!
