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Silanization and BSA coating
Surface treatments like silanization were seen to slow down discoloration in the past. I carried out liquid amino-silanization (protocol in place) of samples from W683 (no RTP) and used them in both 2D and 3D format to monitor discoloration in DMEM(-FBS) @ 37C in Oven. Also BSA coated samples were used side by side for…
Humidity is Affecting PDMS-pncSi Bonding
ByMike HA few weeks ago Dave dropped of a humidity monitor for the third floor equipment room. Since then I have been recording the humidity every time I run a UV Ozone process for PDMS-glass or PDMS-pncSi bonding and there seems to be a correlation between the two. Over the last week there seems to be…
Review of ACKD nanotechnology articles (Vol 20, issue 6, Nov 2013)
This is a cursory review of the nonotechnology articles appearing along with our paper in the Journal “Advances in Chronic Kidney Disease” Most of these are reviews and most deal with nanoparticles for either imaging or therapy. One does discuss nanoparticles for use with membranes to improve biocompatibility. I do not have full access to…
Histograms of recent membranes
Using Mike B.’s MATLAB script, I was able to generate some histograms and stats for wafer 187, 310, 319 and 320. I excluded the “small pore background”, as I do not believe these features go all the way through the membrane. wafer 187 wafer 310 wafer 319 wafer 320 The reason why I did not…
BSA-Au Separations
The BT method mentioned in this post seemed to give stable particles, but the BSA-Au did not work in separations. After about 24 hours, the particles retained pink color but seemed to settle in tubes. This may be due to reversable flocculation, not aggregation. The retentate and filtrates in separation experiments both lost all color,…
Final BBB TEER results
This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells. For all experiments, I used 50000 cells/cm2 as the seeding density. I always had 3 samples – bEnd3 alone, NG10815 alone and the…
