Nanopocket Membrane Fabrication-Update
The Gaborski lab has been working with Blanca’s lab performing some scoping tests w/ SiN and SiOx microporous membranes. The aim is obtain proof of concept results regarding the efficacy of using a microporous membrane to trap sub-micron particles with DEP. Last week, we tested w/ our 200 nm polycarbonate beads. We were able to…
I have been using MatLab to analyze the pores in the SC wafers. Here are the results from my analyses: To take a first look, I simply plotted four characteristics: average pore size, porosity, cut-off pore size, and mean roundness against the wafer number. Notice that the blue dots are the b wafers. Excel would…
In my recent tenting posts, the membrane has shown that it can deform and wrap around edges. I wanted to follow up on this phenomenon and see if we could get a contiguous sheet of membrane around a PDMS edge (not completely sharp). If we can get some membranes around a ~90 sidewall, we could have…
Background Hemolysis is the leading cause of unusable laboratory blood samples; accounting for approximately 55% of all unusable samples [1]. Hemolysis can affect lab results by interfering with light absorption of spectroscopic tests, or by releasing analytes into the plasma sample resulting in a false reading [2]. The syringe used to draw blood from a…
This paper parallels my efforts in creating a microfluidic device that can perform co-culturing of different cell types, allow real time phase and fluorescence imaging, and perform non-invasive TEER measurement using transparent electrodes. This is almost exactly what we aimed for; the only difference is in the type of the membrane. They are using a…
Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and DI or PBS. We are testing for what will make the most optimal cell culture…