Modular assembly of the µSiM

In my last post, I presented the results of my BBB TEER experiments.  For the BBB co-culture, there was a spike in TEER.  I hypothesized that this was caused by media acidification by rapidly proliferating glial cells, which in turn negatively affected barrier function of endothelial cells.  When I presented this data to Angela Glading,…

I’ve taken fluorescence measurements with the fluorescence microscope on PEGylated and unPEGylated glass coverslips and SiN chips. Six coverslips and six chips were prepared. Samples were treated exactly the same, save for the fact that half the samples had PEG Silane in their treatment and half did not (though they were still bathed in toluene…

Let’s get a blast-from-the-past and go through a bunch of drawers in the lab!  I spoke with Jim and it seems like it might be best to schedule it during next regularly slotted Tuesday NRG mtg.  So let’s plan on meeting downstairs in the lab on 2-19 at 9:45 am. Also, I bought an assortment…

PhD Dec. 15, 2010 Thanks for Everything!

This post offers an update on membrane fabrication through nanosphere lithography (NSL), describing in more detail the releasing step as well as the membrane integration into PDMS devices. Membrane lift-off is achieved by dissolving a ZnO film underneath the membrane. After fabrication the sample is immersed in 1M HCl solution. The figure below shows a…