I am trying to build a helpful spreadsheet that outlines all the experiments perfomed with a particular wafer. It also lists the conditions and quality of the wafer. Any other suggestions would be helpful.
To view the spreadsheet, click here.
Jim and I were wondering if we could “image” the membrane windows with the Tecan. A nice thing about the Tecan is that you can specify the number of measurements per well. For example, in this study, I acquired a 10×10 matrix of fluorescence intensities in a “filled circle” pattern (see below). You can also…
Last week, I did another two wafers with different top oxide thicknesses to verify my hypothesis about the top oxide thickness effect on pores. The wafers were 20/15/03(bottom/middle/top) and 20/15/40. According to the hypothesis, the porosity of 20/15/03 would continue decreasing, where for the 20/15/40 structure, the porosity might drop. Same tip I used before,…
A few weeks ago, professor Mike Aziz in Harvard University helped to annealed two samples and in-situ imaged the early stage of pore formation using their Jeol2010-hot-stage-TEM. The oxide seemed intact during the process. The sample was unannealed 20(bottom)/15(a-si)/10(top) three stack with removing the substrate. The first sample was heating to 693C. They took one…
As a proof of principal experiment, we wanted to test if Kilean and I could extract RNA from exosomes following their capture on 1298 membranes. As noted in Figure 1, we first used ultrafiltration to capture exosomes on the nanoporous membrane. We then attempted exosome lysis and RNA release by flowing the lysate below the…
Sugiura 2010 (Anal. Chem) This paper details a PDMS microfluidic system to perform dose response assays (what I’m hoping to achieve over this summer). They use serial dilutions to get separate trials spanning six orders of magnitude, showing an optimal dosage to kill HeLa cells. Their system is very efficient, using very shallow channels and balancing the…
Before Anant left for India, we were doing cell adhesion studies with pnc-Si transwells and getting some weird morphologies. Check it out here, here, here, here, here. This is in contrast with the 2D studies that Anant did for the cell culture paper in which cell adhesion looked good. The main difference is the transwell…
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what happened to the database?
this is a good temp. solution, but we should try to resurrect the effort of an online data store; esp. since we have so much data from different sources now!