Gene vs Protein nomenclature
Human gene/allele/trait: all caps, italicized.
Human protein: all caps (no italics).
Mouse gene: First letter caps, all italicized
Mouse protein: First letter caps, not italicized.
Human gene/allele/trait: all caps, italicized.
Human protein: all caps (no italics).
Mouse gene: First letter caps, all italicized
Mouse protein: First letter caps, not italicized.
Goal: Measure SU-8 shrinkage that have gone through various soft and hard bake conditions To measure the shrinkage, the SU-8 scaffolds needed to be lifted off, but there was some issues. Originally, the SU-8 was spincoated onto an oxide layer, which would be etched away using BOE. The SU-8 had appeared to be disintegrating due…
I’ve been working with the Corning transwell inserts and our SepCon units a bit recently. The addition of the Dremel last week has allowed better control while cutting the transwells, even without the right angle attachment (which will allow cutting in the “drill press” mode). I attached a picture of the process and final unit….
In order to test the sealing of the capillary tubing the PDMS I made a batch of PDMS with a recess in it (from a dime). Once this cured I used the UV Ozone process (@25C) to bond the PDMS to a glass microscope slide. When I arrived this morning the system was fully bound….
Competitive ELISA assay to measure the HA shedding from HUVEC monolayer Introduction The endothelial glycocalyx is a dense layer of proteoglycans, glycoproteins, and glycosaminoglycans (GAGs) that helps maintain the integrity of the endothelial cell (EC) barrier.1 The main GAGs are heparan sulfate (HS), chondroitin sulfate, and hyaluronic acid (HA), among which HS and HA are…
I talked about this yesterday in lab meeting, but here is the data. Since the components of HBSS by themselves did not cause discoloration (HBSS components), I adjusted the concentrations of NaCl, KCl, glucose, Na2HPO4 and NaHCO3 to match that of DMEM. The control was a wafer in DMEM/F12 with 10% FBS. None of these…
Two high school students, Nick and Nikita, were supervised to study the effects our Sepcon membranes had on potential charge based separations. Using standard 30 nm cutoff Sepcons and the pressure cell, they performed a set of experiments with high (1 M) and low concentration (100 mM) KCl. They measured the conductivity of the filtrate…