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Neither Centrifugation, Sonication, nor Filtration Fix the Aggregation in the Barcikowski Lab Samples
In early May, the Barcikowski lab shipped us four samples of gold nanoparticles for us to run separations on. They took forever to get here, and had aggregated and precipitated by the time they arrived. About a week ago, the lab sent another batch of four samples (which differed slightly from the samples they sent…
Investigating vacuoles on transwells
One of the themes in my recent posts has been the presence of ‘vacuoles’ over free-standing pnc-Si and PET membranes. We’ve assumed these are vacuoles because endothelial cells form vacuoles, they don’t stain with live cell stains (and so have no cytoplasmic enzymes) and there can be several of these structures in each cell. In…
Permeability and burst pressures data for new wafers
The permeability data is very different for films deposited at room temeperature and 400C.The 400C deposited wafers have much higher permeability and more consistent. w335 – RT w336-RT w338 -400C w340 – 400C Burst Pressures
TEER Measurements First Attempt
After integrating my thin film electrodes into the layer stack of the BBB device, I want to make some TEER measurements. I stripped off the well-plate attachment off of the TEER tool, exposing the individual leads. To test that the circuit functioned properly, I placed the leads in DI water and then…
Record of light transmitting through the waveguide across the porous membrane
ByJingkaiFrom the last post, I had successfully manufactured waveguide on top of our porous silicon nanomembrane chip. However, the proof that light can transmit through the waveguide was not accomplished until last week. The problems I was experiencing included insufficient thickness and wrong cladding layer refractive index. Thus, in order to prove that my waveguide…
TEER Testing BBB Device v2.4 with Shrink Wrap and Media
After some changes to my fabrication process, I can report a qualified successful TEER test with media (HBSS) in both channels. I utilized a shrink wrap method of fabrication as seen in a previous post. The test was not perfect because I ruptured the channel while moving the fabricated device to a microscope slide. Also,…
