HUVEC Growth on Si: Take 2 and 3
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test. The results of the third growth experiment is shown below.
After 86 hours the cells in the culture wells are confluent (28,000 cells/cm2), with the cells seeded on the glass coverslips and Si growing steadily. The best fit lines are exponential growth according to N=No*exp^(rt), where t is time in hours. R values for cells growing on plastic, glass, and pnc-Si are 0.0278, 0.018, and 0.0249 divisions/hour (.6672, .432, and .5976 divisions/day).
I also tracked discoloration, shown below.
Discoloration of both RTP and non-RTPed samples occurred more quickly than shown in Anant’s post. This may be attributed to the processing/properties of W334 (Cu contamination) compared to W311 and 312.
Can you plot the cell growth as semi-log y.
Fixed.
OK now normalize to the initial values. Sorry should have thought of that last time.
Now calculate the per capita growth rate.
The slope of the tissue culture plastic is surprising. Were the cells getting close to confluent on the TC dish?
Was RTP in AR or O2?
In Ar.