Sample buffer adsorption assay with pnc-Si
Previously I have shown that sample buffer can be used to lift adsorbed proteins off polymer membranes, which can then be imaged on a gel. I have used a similar procedure to assay adsorption on pnc-Si membranes.
I used 4 samples from wafer 216 that all had pinholes. I incubated a drop of 1mg/mL BSA on these membranes for 20 min. I rinsed the membranes in dH2O and placed a 30uL drop of sample buffer on them. I removed the sample buffer after 1 hour and ran the samples on the following gel.
While not quantitative yet, these results show that comparison will be possible. This gel cannot be normalized to the previous one, but superficially it looks like less BSA adsorbed to the surface. To enable comparison I will have to run these gels with known mass standards. One other thing to keep in mind is that I’m assaying adsorption to the entire surface of the chip and not just the active membrane. The final figure will look like a bar graph (with multiple samples and error bars).