Diffusion Cell: blood protein
Last week Jess helped me set up the diffusion cell with human albumin and cytochrome c. We ran 3 cells and used membranes from wafer 565. The membranes used were -1,4 (retentate 1), 3,3 (retentate 2), and 4,2 (retentate 3). The gel is posted below:
The columns are (from left): Protein, control, retentate 1, filtrate 1, retentate 2, filtrate 2, retentate 3, filtrate 3. All 3 filtrates show that some of the proteins passed through. The retentate on the well side of the membrane evaporated overnight. We put PBS on the well the pick up anymore of the retentate that was stuck to the well.