Sepcon-transwell discoloration study I
In an earlier post we saw that intact membrane sample discolored slower than the broken one. To establish this, a study using four types of samples from #702 (blank chip with no slits, chip with both intact membranes, chip with one intact membrane and chip with both broken membranes) was carried out.
The samples (no RTP) were all in Sepcon format with membrane side down, in DMEM (-FBS) @ RT.
According to our hypothesis that ‘well side’ triggers the discoloration of the ‘membrane side’ and thereby its degradation, we would expect the discoloration to be in the following order (faster to slower): both broken membranes > one intact membrane > both intact membranes > blank chip with no slits. This is after considering that presence of membranes in Sepcon format helps in separating the two sides of the chip (well side & the membrane side) and thus slows down the discoloration.
In my first trial, I didn’t get the expected trend, as can be seen in the figure below.
But there seems to be a trend…
I will repeat this experiment with a different wafer (since #702 was found to discolor faster than usual) and in oven (37C).
Are these repeats, or is it day 1, day 2, day 3 in the columns from left to right? If these are repeats, I wonder why there is so much difference in the 1 intact membrane samples? These are some really interesting coloration patterns.
These are all repeats. I had planned to take samples for different time points but there was no discoloration after 6 hours and 24 hours. I couldn’t check it on day 2 and saw (both intact slits) samples to discolor completely on day 3. So, instead of having different time points, it turned out to be repeats for all.