Carbonization and Discoloration, final time point
A few weeks ago, I posted discoloration results on carbonized membranes here. It was 6 weeks yesterday since I started this experiment, so I decided to stop it (few, if any, cell applications require 6-weeks of culture). These were SC141 chips in the flat (not transwell) format. I also used DMEM with high NaHCO3 levels. Therefore, these are the harshest cell culture conditions.
The previous post stopped at 3 weeks with 1 chip slightly discolored to dark purple. After 6 weeks, that chip completely discolored, but the other chip was normal. I’m not sure how to explain the difference but I think it’s safe to say that carbonization dramatically increases pnc-Si stability in cell culture conditions.