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Since HUVEC morphology hasn’t looked good in short-term adhesion assays recently, I analyzed HUVEC on the 2nd day after cell seeding. Normally, CMFDA staining is done for 30-45 minutes in serum-free media.  I wondered if the serum-free environment during staining was affecting the HUVEC.  So, I stained one sample with 5uM CMFDA in serum free…

I attached a presentation here for tomorrow’s meeting. sensor

In a previous post I had shown the relative fluorescence of various substrates as normalized to glass.  1um beads (either green or red) were placed onto each surface and imaged with constant EM Gain, exposure time, and intensity.  Bar graphs were constructed to show the relative fluorescence (red and green were not normalized to each other)….

We have re-started the PEG x-linking project at RIT. One of the goals is to create microwells made of PEG on top of a membrane in order to culture individual cells or small colonies of cells with the potential for a co-culture with a confluent monolayer on the opposite side. PEG is available as PEG-Diacrylate…

Two experiments were performed to explore application of SiMPore filters within INT’s microfluidic cartridges to allow the capability for pathogen type specificity in nucleic acid purification. 1) The first experiment tested the efficiency at which nucleic acids could pass through the membranes. T4 polynucleotide kinase was used to 32-P radiolabel two different sized PCR products:…

  Overview I burst pressure tested TEM chips with 1×1 mm windows of 100 nm SiO2 that were both annealed and unannealed (provided by JP and Josh Miller). In our experience it seems that the forming gas (H2/N2) annealed chips are stronger and never wrinkle. These burst pressure tests were performed in an attempt to…