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Drifting and Biasing Potentials in the Electrodes Make Streaming Potential Measurements Difficult
A few weeks back I had finished my streaming potential/electro-osmotic pump setup. It looks like this: For streaming potential measurements, we pressurize a tube filled with 100mM KCl that connects to the membrane and measure the voltage drop across the membrane. When I first applied a pressure to the setup, the voltage drop did seem…
Additional Diffusion Assays
Cellulose Membranes I set up a 24 hour diffusion experiment with Millipore 30kD cellulose membranes as shown in this picture: After 24 hours I collected retentate and filtrate and visualized on the following gel: I didn’t expect to see a separation so similar to our pnc-Si results in this format considering the extremely poor dialysis…
Combining Pores Affects the PoreImageProcessor, but Not Enough to Throw Permeability Off 2x
In Tuesday’s NRG, we wondered if the fact that some of Josh’s pores overlap could throw the PoreImageProcessor off enough to explain a 2x discrepancy between our theoretical hydraulic permeability and our experimental permeability (the experimental was lower). From this picture: I took the following close up: If we assumer that each of the sub-pores has a radius of…
AFM – new tips and wafer characterization
I ordered some new AC160 tips last week from Asylum and got on the AFM Thursday to test them out. I think they solved some of the problems I was running into over the last few weeks. Mainly, I was finally able to image entirely in repulsive mode (which was impossible with the older tips). All of…
Water Permeability and Pore Comparisons
Previous Post on Water Permeability Figure We decided at the last meeting to show water permeability for each membrane used rather than plot for a specific characteristic like porosity. I did this but there were a few samples that still had a noticeable deviation between experimental and calculated permeabilities. I asked Dave to reprocess the…
Progress in Modelling Sepsis: Diminishing PMN chemotaxis towards fMLP gradients in µSiM Devices
First, a quick publication update. Recently we’ve published a paper on our efforts towards computationally analyzing polymorphonuclear leukocyte (PMN) trafficking on a vascular endothelium via hand-built µSiM flow cell devices. We record videos in phase contrast with a 40x objective and are able to delineate PMN state, speed, and persistence by leveraging ML algorithms and…