Shear Free Chemotaxis Manuscript (Submitted to LOC)

A few weeks ago, I posted discoloration results on carbonized membranes here.  It was 6 weeks yesterday since I started this experiment, so I decided to stop it (few, if any, cell applications require 6-weeks of culture).   These were SC141 chips in the flat (not transwell) format.  I also used DMEM with high NaHCO3 levels. …

Cindy Hartnett – professor of mechanical engineering at Louisville requested some pnc-Si chips after reading our PNAS paper. In requesting the material she mentioned … I’ve long been searching for a low-voltage way to drive some micro-actuators. Electrokinetic pumps looked good but until now the necessary voltage was far too high. About a month after…

Last week Jess helped me set up the diffusion cell with human albumin and cytochrome c. We ran 3 cells and used membranes from wafer 565. The membranes used were -1,4 (retentate 1), 3,3 (retentate 2), and 4,2 (retentate 3). The gel is posted below: The columns are (from left): Protein, control, retentate 1, filtrate…

I got some new data on the pnc-Si capacitive sensor that has not been published on the blog yet. All of the results are summarized in the Spring MRS Proceedings paper mrsspring09_kavalenka. I also uploaded presentation that I made on MRS in “Presentations ” section of the blog. I will talk more about this data…

Even though we don’t have defect free membranes, I wanted to tweak the gold setup using membranes with pinholes. Previously I was using the small scale diffusion chamber that I use for protein separations. This setup has a retentate of 2-3 uL and a filtrate of 60 uL. To do particle sizing using the Malvern…