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Serial Dilution of Rhodamine
I was having trouble using the spectrometer to measure rhodamine concentration in the stir cell. I ran a standardized absorbency of the serial dilution of rhodamine. The curve is posted below. It appears to be linear. The molarity of rhodamine must be above 2uM in order for the spectrometer to read it. I think I…
PLA Plastic Acidifies DMEM + 10% FBS
I have been looking into PLA (Poly Lactic Acid) plastic for use in our microfluidics. PLA is a commonly used material in 3-d printers, so it would be great if we could print out our microchannels for cell-culture devices. To that end, I wanted to see if the PLA plastic would react with our…
Top SiO2 Thickness Experiment (3)
ByJoe QiLast few weeks, I deposited a couples of wafers to test the effect of oxide thickness on pores. In addition to previous data, I deposited another two wafers, one with 1nm top oxide and the other with 100nm top oxide. I summed up all data from the top oxide experiments. The first image is the…
New HBSS
I decided to find another supplier for HBSS and found one: CellGro. CellGro HBSS had the same components as Gibco HBSS, although a couple of the concentrations were different. I tested out this new HBSS vs. homemade HBSS. Here, the commercial versions of HBSS caused discoloration within a day, but my homemade HBSS didn’t. The…
Wafer 404
Last week I reported wafer 403 showed uniformity across all positions but had a huge amount of pinholes. Wafer 404 (TEOS process & thermal oxide base) was initially annealed at 1000 C and then annealed again at 1000 C for 5 minutes in the RTP. The second anneal helped with the sputtered oxide film quality…
CytoVu Static TEER Device with Yuchen Wang
Yuchen and I have been tasked with integrating TEER electrodes into the Cytovu format. The general idea was to fabricate the TEER electrodes similarly to what I have done already, then add them to the current Cytovu device, using UV ozone bonding and PDMS (to cement in the top electrode). I drew some electrodes to…