Similar Posts
Side-dependent barrier function
I’ve suspected that the barrier function of endothelial cells might be surface-dependent (that is, cells grown on the pnc-Si side vs. cells grown on the well side). I hypothesized that the interface between membrane and well walls would alter the continuity of the endothelial monolayer when cells are grown on the well wide. To test…
BBB co-culture (2)
In my last post (the first co-culture), I showed that bEnd3 cells exhibited a different morphology when they were co-cultured. I also decided that I would reverse the orientation of the co-culture in order to better visualize the endothelial barrier. That is, grow bEnd3 on the basolateral side and NG10815 on the apical side of…
Flow Cell/µSiM Testing – Push versus Pull Flow Modes
Introduction Static endothelial cell cultures have been routinely performed and optimized on the µSiM platform for multiple membrane and cell types. In order to increase the physiological relevance of our device with tissue vasculature mimetics, we need to incorporate fluid flow to emulate shear stresses experienced by endothelium under physiological circulatory conditions. Recently Mehran has…
Getting (lots of) DNA though NPN filters
Warning: the following post contains spoilers for Vincent’s talk at Rochester on Monday. Since the last update, I have tried to get the gold nanoparticles working properly, with no luck so far. The zeta potential we measured in KCl was 0mV, showing that even though we had visual confirmation that there were particle suspended in…
Solid State Nanopores in Biosensing
Papers: Multi-Nanopore Force Spectroscopy for DNA Analysis Control of DNA Capture by Nanofluidic Transistors 2013 SingleMoleculeReview
Cell-Basement Membrane vs Basement Membrane-SiO2 Interactions in a Shear Influenced Device
ByAlecIntroduction When concerning the issue of adherence on any substrate, the main two interactions that matter most are that of the cell interacting with the basement membrane (whether cell produced or artificially added) or the interaction between the protein based basement membrane and the substrate providing structure for cell culture; in our case SiO2 membranes….