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Who’s Your Cabby? Analyzing the Roles of Haptotaxis and Durotaxis on Staphylococcus Aureus Invasion of the Osteocyte Lacuna-Canicular Network
As discussed in a previous post of mine, I am currently working on a project to develop an in vitro mimetic of the osteocyte lacuna-canicular network (OLCN) to improve the ability to predict Staphylococcus Aureus invasion and colonization of the OLCN during osteomyelitis. The purpose of this post is to provide more background behind Aim…
Hemocompatibility of pncSi – Complement Activation Studies
This is the third set of studies to assess the hemocompatibility of pncSi. It aims towards a quantitative analysis of the formation of the complement protein C3a in the blood upon interaction with pncSi surfaces. Biological Motivation: Complement proteins help the functioning of the innate immune systems in clearing away the pathogens from the organism,…
Peclet Number Analysis Update
ByKileanWith changing my channel height to attempt to lower the Peclet number, one important factor that is affected is the transmembrane pressure. Decreasing the channel height will significantly increase the transmembrane pressure, which may stress the membrane and cause it to break. However, in addition to changing the channel height, I will also be changing…
I-V characteristics of coated membranes
ByJimI measured the I-V characteristics of our coated membranes on Wed. at RIT. JP observed the measurement. The semicon parameter analyzer in the test lab is “old” but isexactly what we need! Roughly speaking, our coated membraneshave resistance about 2k Ohm, depending on the thicknesses ofmetal films (this is based on pure Ti coated samples,…
RPE ZO-1 expression observed on MgF2 and tissue culture plastic
Here are some more images from my ongoing cell culture project. My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates I added twice the amount of primary antibody on these devices that I usually do (1:100 dilution, instead of 1:200). High Porosity MgF2 TCP MgF2 Coverglass
Hindrance is magnified in thicker membrane
Using the same procedure as in the last post, I was able to simulate a 6um thick membrane with the same pore characteristics as the experimental pnc-Si membranes. Material diffuses more slowly through this membrane, but not as slowly as I expected. The following figure shoes the filtrate/retentate concentration ratios at 24 hours for a…