Similar Posts
Culture divider insert assembly and test
Introduction We are designing a cell culture chamber insert to allow side-by-side co-culture of cells and ultimately triculture in the µSiM devices. Materials and Methods ALine devices assembly according to the “ALine Modular Device Assembly” instructions. 2-slot NPN membranes applied (NPSN100-2L, nanoporous silicon nitride 100 nm thick, 2 windows). Straight tweezers and chip tweezers are…
iPSC-derived Brain Pericyte-like Cells
Introduction Pericytes are mural cells that line the outer surface of microvessels and regulate diverse aspects of vascular development and function. We differentiate brain-pericyte-like cells (BPLCs) from hiPSC clones using the protocol published by Eric Shusta’s lab (Gastfriend et al., 2021) (Figure 1). This protocol describes methods to differentiate hiPSCs to BPLCs via neural crest…
Introduction of beads into the flow module
1. Introduction Fluid flow is one of the key elements of vascular models for delivering nutrients and growth factors to cells as well as introducing other components like neutrophils. In post 1, we described our modular design for incorporating a flow module into the uSiM device. Then, we presented fluid flow simulation and its experimental…
Manufacturable Single-Slot Flow Insert Update
Introduction A recent collaborative publication from RIT developed a modular µSiM to address the lack of physiological fluid flow in conventional open-well devices (Figure 1). The magnetic latching assembly of this design allows for quick configuration into a fluidic setup. The flow module is a microfabricated PDMS insert that contains a channel over the membrane…
Theoretical Underpinnings of Small Molecule Permeability Measurements in the µSiM (Part 1: Approach)
ByJimNOTE: Some of the ideas in this post are borrowed and rephrased from prior posts by Molly and Alec and our paper Khire et al. 2020. The purpose of this thread is to explain things anew with an eye on application in the R61 project. Small molecule diffusion from the well to the channel side…
Multiplexed digital assay: mouse CCL2(MCP1) CXCL1(KC) panel development
Mouse CCL2/MCP1 and CXCL1/KC pre-equilibrium digital ELISA has been developed with the current dynamic range set to be 4-500pg/ml for CCL2/MCP1 and 8-1000pg/ml for CXCL1/KC. This initial dynamic range was selected by inferring from the ELISA kit reagent working concentration given by the vendor R&D systems (1.9-250pg/ml for CCL2/MCP1 & 8-1000pg/ml for CXCL1/KC) and can…