Elder Lab Operation Clean Glass
Elder Lab Operation Clean Glass
Materials
Biological Safety Cabinet (BSC)
Ultrasonicator P250 T
0.5 or 1 L glass Beaker
ddH2O (Barnstead 18 MΩ)
100% isopropanol
Alconox Cleaning Solution
Glassware, e.g., Petri Dishes
Aluminum Foil
Oven that can reach 250°C
Gloves (nitrile)
100% Cotton Lab Coat
- Preparation
Put on cotton lab coat and nitrile gloves and cut pieces of aluminum foil ~4in x 4in and place them on an oven rack that is covered with a large sheet of foil. Bake at 250°C for 5 to 10 hours, allowing an additional time for the oven to reach working temperature after items are placed inside. When ready to use, stack them and place in a beaker covered with foil for transport.
- Clean Glassware
Place all the glassware into the beaker and submerge in ~5g/L of Alconox and get everything soapy but gently swirling the Petri dishes in the water. Decant and fill with Barnstead ddH2O, repeat 3 times by rotating the beaker as it is filled. Fill the beaker with 100% isopropanol, submerging the glassware, and sonicate 3-5 minutes with a fitted aluminum foil lid. Immediately wrap them with pre-baked aluminum foil as the pieces are taken out of the IPA, snug enough to cover all the surfaces but loose enough to allow the alcohol to evaporate. Wrap all the pieces together in one sheet of foil and transport them to an oven set at 250° C and bake for at least 5 hours. Let cool overnight.
- Storage
Clean glassware is left in aluminum foil until ready for use; it can be stored in a pipette tip box in a BSC. Petri dishes with samples should always be sealed with parafilm and wrapped in aluminum foil.
