The current SPTF team at RIT (Eddingsaas, Hernandez, Routenberg) has been working on blank testing of proposed analysis workflows to extract < 53 µm microplastics from lake water samples, with a goal of ensuring low contamination levels in the workflow prior to analyzing field samples. Field water samples were collected from Lake Ontario throughout 2025…
Elder Lab Staining Airborne Microplastics Materials Biological Safety Cabinet (BSC) Side arm flask, 150-1000 mL Small pin Fritted glass filter Vacuum and tubing 0.5 µm Sepcon Filters 1.7 mL microcentrifuge tubes Microcentrifuge ddH2O (Barnstead 18 MΩ) 100% isopropanol (IPA) 100% Ethanol (EtOH) 70% Ethanol (EtOH) 0.4% Gibco Trypan Blue (TB) – 1 year shelf life…
Elder Lab Operation Clean Glass Materials Biological Safety Cabinet (BSC) Ultrasonicator P250 T 0.5 or 1 L glass Beaker ddH2O (Barnstead 18 MΩ) 100% isopropanol Alconox Cleaning Solution Glassware, e.g., Petri Dishes Aluminum Foil Oven that can reach 250°C Gloves (nitrile) 100% Cotton Lab Coat Preparation Put on cotton lab coat and nitrile…
This post is about how I prevent contamination in the lab during experiments and material preparation. Contamination has been an issue in sample preparation despite efforts being made to minimize contamination. Current efforts include wearing cotton lab coats across all projects and the MMC, triple rinses with tap water followed by triple MilliQ water, working…
This post is to go over how to post a blog post. This post is written like a protocol, but you can do anything! It is meant to enable live data uploading and review by others. Step 1: Log in to the nanomembranes.org website (or trace-bmps.org for TraCe users). If you are reading this blog…
Introduction The LOMP Small Particle Taskforce is focused on developing a reliable and rigorous procedure for analyzing small microplastics in environmental samples. Our primary aim is to ensure that the data we generate accurately reflects the true microplastic population present in each sample. Achieving this requires careful attention to representativeness, verification, and contamination control throughout…
Introduction The LOMP Small Particle Taskforce is focused on developing a reliable and rigorous procedure for analyzing small microplastics in environmental samples. Our primary aim is to ensure that the data we generate accurately reflects the true microplastic population present in each sample. Achieving this requires careful attention to representativeness, verification, and contamination control throughout…
Introduction The LOMP Small Particle Taskforce is focused on developing a reliable and rigorous procedure for analyzing small microplastics in environmental samples. Our primary aim is to ensure that the data we generate accurately reflects the true microplastic population present in each sample. Achieving this requires careful attention to representativeness, verification, and contamination control throughout…