I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells. Here’s a post of images. I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve been investigating the optimal arrangement of the different cell types (bend3 on top or bottom?). …
I was motivated to scan our membrane edges after looking at this image from the paper Maryna presented last week: I found a couple of pnc-Si samples with membranes still attached to the edges. I also found one sample with a membrane split almost down the middle. I coated the samples with 3nm of Au…
This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells. These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days. Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but one survived this 7-day cell culture experiment. These cells were plated on the bottom on…
In my previous post, I showed images of bEnd (P8) cells on PET and pnc-Si transwells. These were seeded at 105 cells/cm2 and grown for 14 days. This post contains the TEER data. I simply normalized all values to the Day 0 (no cells) resistance, so everything starts at 100%. This data for PET matches…
I continued to run some of the samples that I described in this post last week. These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS. The pnc-Si was SC126 that was RTP’ed. I measured the TEER of these samples for 2 weeks and then stained the cells…
With the previous 2 experiments, I made a couple of 6-slit pnc-Si transwells. These chips were from the cell culture wafers I had made a while ago. These samples (SC126) were RTP’ed before use. My improved technique, combined with the RTP, enabled me to keep cells growing on these pnc-Si samples for 1 week WITHOUT…
Along with the PET data I just posted, I also examined cells on pnc-Si transwells. The following images were taken at 20X (through the bottom of a 24-well plate), and the cells are passage 8 bEnd3. They were seeded on the membrane side @ 100000 cells/cm2, and then the transwells were flipped for growth. Images…
A while ago, I posted some images of cells grown on commercial polyester (PET) transwells. Here’s the post. I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the bottom of the membrane. I contacted Corning and they said that these dead cells were…
I took some force curves a while ago to see if there was any difference between wrinkled and taut membranes. Here, I present only the data with a 15nm ‘trigger’. The trigger is a user-defined parameter that sets the maximum deflection of the cantilever during a force curve acquisition. THe y-axis in these graphs is…
I’ve been thinking of demos/experiments to do with my upcoming class and one of them is SEM. I was thinking of handing out some unknown nanoparticle solutions and have them figure out the size and maybe material by SEM, AFM and/or DLS. I’m approved to run the SEM now, so I hopped on today to…