This is a follow-up post to “Protein Assay Test.” I realized that I never posted the final stag of my experiments, in which I finally got BSA to go through the membranes. In “Protein Assay Test,” I described several experiments that I set up to see if I could get BSA to pass through a…
The purpose of this post is to see if there is a pattern in the Pore Density vs. Pore Radius graphs at certain manufacturing conditions. The following wafers all have widths of 15nm and RTPs of 1000. The porosities range from 6.48% to 8.33%. **** note: the y-axis label should actually read “pore density (#/nm2)”…
Over the past few weeks, I have been trying to separate proteins with the SepCons. As a preliminary test, Barrett and I decided to first acertain that BSA could pass through a membrane with pinholes. Theoretically, the pinholes are so large that the BSA should easily pass through. However, it doesn’t appear that the BSA…
This post is a follow-up to Professor McGrath’s post, “Permeabilities vs. pore sizes for ideal membranes.” In the aforementioned post, this equation is proposed: Ψ = Nπr2 Where Ψ is the porosity, N is the density of the pores, and r is the pore radius. Rearranging the above equation (assuming a constant porosity): Ψi =…
I went through and characterized each wafer by thickness and RTP. I graphed “Average Pore Size vs. Porosity” and “Average Pore Size vs. Cut-Off Pore Size” for each processing condition that had at least four representative wafers. ** Yesterday, Professor McGrath and I had a quick chat about the data. I have inserted a few…
Roundness vs. Equivalent Diameter plots for five eccentric wafers: There really doesn’t seem to be a correlation between equivalent diameter and roundness. However, there does appear to be a rough downward trend in SC 016 and SC 258. Mean Roundness vs. Wafer Number graph with error bars included: Without the error bars, it looked like…
Here are the updated pore processing graphs: The data spreadsheet: pore-processing-results Supplement (added 6/16/09): Here is the Average Pore Size vs. Porosity graph with the outlying data points labeled: After making this graph, I looked at the outliers to see if they were due to my errors. A few were and I redid them, updating the…
I have been using MatLab to analyze the pores in the SC wafers. Here are the results from my analyses: To take a first look, I simply plotted four characteristics: average pore size, porosity, cut-off pore size, and mean roundness against the wafer number. Notice that the blue dots are the b wafers. Excel would…
I have been working with Barrett to test two different assays with BSA. One is a standard procedure for microtiter plates and the other is a microassay procedure for microtiter plates. Here is a brief summary of each procedure: Standard Procedure for Microtiter Plates– linear range is 0.05 mg/ml to 0.5 mg/ml Prepare a diluted…