What process flows have I used to create my devices? Standard Cut out gaskets (10 min) Embed Electrode (face down) (3 hrs) Ozone Bottom, Interstitial, Filter and Top channels together (1 hrs, cure overnight) Ozone Bottom Electrode (1 hr, cure overnight) Ozone Top Electrode + Feet (1 hr, cure overnight) Weaknesses are that there are…
Dr. Webb is very interested in the thin nanomembranes that our group experiments with. To date, he has been trying to get cells to grow on SiN microporous material and image them with a few different types of microscopy (Phase, Confocal, Scanning Conductance Ion Microscopy). The cells have not grown well natively on the nitride…
I created some quick open air devices to test the viability of different adhesion molecules. The basal well has a volume around 150 uL, and the apical well has a volume around 15 uL. I made 6 devices in this format. On 5/14/13, Alan and I filled the basal chamber with PBS and then…
I found this article about a macro-microfluidic interconnect that I want to try out at some point. We might even be able to seal tube-block interface with PDMS for a tighter seal. http://blogs.rsc.org/chipsandtips/2006/11/13/a-simple-interface-between-a-pdms-microfluidic-device-and-a-syringe/ A simple interface between a PDMS microfluidic device and a syringe 13 Nov 2006 By Francesca Burgoyne. Jeonggi Seo and Meng…
I made a lot of 6 devices for Vaccinex. We attempted to seed human endothelial cells on the membranes, flowing at 20 uL/min, first coating the surface of the membrane with an adhesion molecule at 20 uL/minute. At the same time, the same cell line was grown in a 24 well TEER z-scope tool to…
I took some copper tape and made a bus bar for BBB device V2-8. I used tweezers to smooth down the surface of the tape on each planar surface, routing the copper to the back side of each face, then collecting the lines to one side of the device. I verified that the connections…
As the device animation shows, the layout of the chip has changed. The incorporation of through-vias in the bulk silicon allows me to eliminate a gasket wrapping around the filter, which was a large source of leaks. I used ozone bonding for 8 minutes for each layer to join the device together. I initially taped…
BBB Device V2-8 Filling Up by the way, the code to embed .mp4 videos uploaded to the server is: ” <video width=”640″ height=”360″ controls> <source src=”http://example.com/wp-content/uploads/video.mp4″ type=’video/mp4′></source> <source src=”http://example.com/wp-content/uploads/video.ogv” type=’video/ogg’></source> </video>”
For the first time, we did time-lapse phase microscopy (1 frame / 5 seconds) looking at endothelial cells through the ITO in the static BBB device (device A bottom left well) and measuring the TEER concurrently. Here’s the video, just shy of 15 minutes of recording. (reporting a low resistance: 5-10 ohms, shorted). TEER Test…
We grew some endothelial cells on ITO and glass slides to see if there were biocompatibility issues. We also put some of the thermally cured circuit writer ink afterwards to see if they were cytotoxic. Two devices were constructed: one where cells were growing on ITO and another device where the cells were growing on…