Yuchen and I have been tasked with integrating TEER electrodes into the Cytovu format. The general idea was to fabricate the TEER electrodes similarly to what I have done already, then add them to the current Cytovu device, using UV ozone bonding and PDMS (to cement in the top electrode). I drew some electrodes to…
Fabricate ITO Electrodes Deposit 2500 Angstroms, ITO, Room Temperature Using Kurt Lesker Sputterer (Use ITO Variable Recipe, takes about 1.5hr) Tape in Microscope Slides and Coverglass into Machined Shadow Mask using Kapton Tape Anneal in Toaster Oven, 450 Fahrenheit, 1hr Drops resistance from > 40 Megaohm to < 5 kohm. Measure from edge of Pad…
Links and Papers: Article with video Lung on a Chip Device Paper Lung on a Chip Application (Pulmonary Edema) Chris came across the publicity video last week and Jim asked me to look into the specifics. It’s a pretty nifty device. As the video shows, the device is a co-culture structure fabricated entirely from PDMS….
BBB On Chip (Griep et al 2012) Jascha sent me an article about a BBB device. Their device follows a very similar structure to that of the Booth and Kim paper, but closer to the optimized design that I created, using very thin, wide channels to generate a lot of shear stress (100 micron high,…
I’ve been thinking a lot about energy storage lately, and I think I’ve found a potential use for pnc-Si in Supercapacitors. What is a supercapacitor? As the name suggests, it’s a double-layer capacitor (EDLC) with very high capacitance values (1-500+ Farads) or a million times more than that of a typical dielectic capacitor. An electrolyte…
Josh has been kind enough to fabricate a shadow mask for me to create interdigitated electrodes without the hassle of photolithography and etching. In order to be able to use this method, I want to make sure the produced electrodes are the same or better than the old method. I worry about shadowing effects due…
Following my fabrication process from my previous post, here are the results from my flow test of BBB device v2.6: The difference in the quality of the electrodes is apparent from these images. The microscope slide electrode was difficult to spin coat, resulting in an uneven photoresist coating, and worse patterning. Etching in 12M HCl…
Given the results of my previous post, I am reworking my design to make it more manufacturable. BBB Device V2-6 Compressed The major change compared to my last design is the inclusion of a full length microscope slide and coverslip. Instead of cleaving the electrodes from the glass, this step eliminates the need to…
To prepare the BBB devices for cell culture, they must be sterilized. To that end, I wanted to see if there were any adverse effects from autoclaving the device. Recipe 4 on the U of R autoclave is a dry process that we use to sterilize tips, so I used the same recipe. I placed…
After some changes to my fabrication process, I can report a qualified successful TEER test with media (HBSS) in both channels. I utilized a shrink wrap method of fabrication as seen in a previous post. The test was not perfect because I ruptured the channel while moving the fabricated device to a microscope slide. Also,…