Last week Dave deposited sep-cons ( cell culture mask) with 3 different thickness namely the regular 15nm , 30 nm and 50nm ( SC 142, 143,144). These wafers were characterized and it was noted that the 15 nm had a high pinhole density and the 30 and 50 nm films were low pinhole density. The…
We’ve been getting some strange cell morphologies recently in short-term adhesion studies. My previous post on this subject links to a few of these results. Unfortunately, my last experiment into short-term cell morphologies on autoclaved and EtOH- sterilized samples wasn’t well-controlled since I didn’t have enough samples. Therefore, I repeated this adhesion study with proper…
Some transwell cell culture models require 1 week or more of cell growth in order to let the cells fully differentiate and form mature monolayer barriers. I had a couple of leftover samples a couple of weeks ago, so I measured the viability of cells on RTP’ed pnc-Si and PET transwells 7 days after plating…
This was the first try at using PES for protein separations by diffusion. 100kD cutoff Nanoseps were used to separate 10mM KCl and 100mM KCl standards. In both cases, the backside fluid was gone after 24hours. I don’t think evaporation was the problem; either the membrane sucked up the fluid or it was drawn up…
With the new heater installed in the AJA sputter tool, the production team decided to run a set of wafers at six different chamber temperatures to re-characterize membrane morphology, mechanical strength and overall quality of material. These films were all deposited with 25 W substrate bias and annealed at 1000 C in the RTP with…
Last week I reported that carbonization can shrink pores. This week I was able to completely occlude the pores with a change in the process recipe. Instead of cutting off the acetylene flow before the thermal treatment, I kept the gas on for the entire process. What resulted was a complete occlusion of the pores….
I reran the samples from this post on coomassie stained gels. The sensitivity is lower, but the results look reasonable. First off I check out how well the sum of the retentate and filtrate matched the starting sample. This was much closer with the 100mM trial. There seems to be more error/loss in the 10mM…
Dave did a series of 20nm SiO2 film depositions at different temperatures (constant bias) and gave them to me to AFM. I guess he wanted to see if film roughness changed drastically over the range of ‘room temp – 400C’ in order to investigate this as a potential source of pinholes. I used a new…
In this gel I evaluated the linear range of coomassie brilliant blue. From this test it looks like things are linear from .0156 to .125mg/mL. My guess is that the protein ladder bands in the previous experiment fall within the range. I used the Bradford assay to find the total concentration of the stock protein…
Previously I have silver stained all gels because the separations look very clear with this staining technique. However, the silver stain is not giving very quantifiable results. Coomassie staining means a loss in sensitivity, but it’s the gold standard for quantifying protein amounts. Here I’ve stained one of the protein separation results and rerun with…