Charged Based Separations w/ Coomassie Gels
I reran the samples from this post on coomassie stained gels. The sensitivity is lower, but the results look reasonable.
First off I check out how well the sum of the retentate and filtrate matched the starting sample. This was much closer with the 100mM trial. There seems to be more error/loss in the 10mM trial.
I also plotted the filtrate/retentate ratios for these two gels:
The 100mM species are much closer to equilibrating at the smaller proteins. I think this still gets the differential separation idea across.