Due to our desire to improve membrane stability in cell culture, different methods of treatment have been mentioned to try, including carbonization and silanization. W606 was treated with UV/ozone at 150C for 20 minutes, followed by silanization performed by some of Chris’ colleagues. I then ran a basic discoloration test in DMEM +10% FBS (the…
Previously W514 was tested with different RTP conditions (varying time at 800C, all in Ar); the results were difficult to interpret as the cells were confluent (in some cases it was hard to see membrane breakage). We therefore decided to run a similar test again, with a slight change in O2 addition during RTP. O2…
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs. HUVEC spreading on pncSi HUVEC spreading on glass
This was something I set up a few days ago and finally got a chance to run the gel. I was curious to try this after the previous forced separations using brain extract. If you recall, the “separation” using the pressure cell did not have a visible cutoff and all of the bands appeared in…
Harold Smith at the Med Center proposed an experiment for Jess and I to try, in which we use our membranes to separate proteins onto a PES, PVDF (Polyvinylidene fluoride) or Nitrocellulose membrane, similar to a Western Blot of DNA microarray. The idea is to create a system to screen for drugs that break protein-protein…
Previously I ran a chemical stability study of samples from W613, which has a 30 nm thick pnc-Si layer. In that study complete failure of untreated samples was observed by 18 hours in cell culture, which was not a significant improvement from sample lifetime of 15nm thick pnc-Si. To see if RTP treatment helped the…
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
Based on the results obtained yesterday with the closed flow cell we reran another trial today. The closed flow system we tested today was bound using UV Ozone treatment, surprisingly this system created a relatively strong bond despite our resent results. Once the UV Ozone treatment was complete and the system had bound vacuum grease…
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on glass 1 Fibroblast spreading on glass 2 Time lapse movies for fibroblasts spreading on pncSi…
We’ve been assuming that porosity follows a linear relationship with respect to wafer position, but this turns out to be incorrect. I imaged membranes from five points on wafer 620 and found that maximum porosity does not occur at the outer most sample, as previously thought, but instead at r=3. Does this provide a better…