μSiM

IntroductionThe ability to continuously sample and assay for the levels of soluble factors without disrupting the microenvironment is important to acquire sequential secretion profiles in BBB experiments on an uSIM module. Combining small volume sampling, buffer replenishment and sensitive pedELISA technology would enable longitudinal assays of proteins introduced to blood compartment or released from cells….

Introduction Sepsis is a serious condition where unregulated systemic inflammation can cause organ failure and death. Recent evidence has also shown that sepsis can cause cognitive impairment in surviving patients. However, the mechanisms that cause sepsis-related damage to the brain are challenging to study with the current in vitro tools. In this project, our goal…

Introduction Human induced pluripotent stem cell (hiPSC)-derived blood-brain barrier (BBB) models have proven to be useful to study disease pathomechanisms and drug delivery into the central nervous system. Currently available hiPSC-derived BBB models are well characterized with respect to diffusion barrier properties and specific transporters, however not optimal for studying BBB-immune cells interactions because of…

Introduction Breakdown of the blood-brain barrier (BBB) is one of the earliest signs of sepsis and is linked with long-term cognitive impairment. As animal models poorly recapitulate human sepsis, alternative approaches are direly needed to understand sepsis-associated brain injury and develop strategies that protect against cognitive decline. To fill this gap, we are developing a…