We have seen that discoloration of chips is slower in Sepcons and this has been speculated to be because of the separation between the ‘well side’ and the ‘membrane side’ of the chip existing in the Sepcon format. To further provide evidence to this theory, I carried out an experiment monitoring the discoloration of samples…
In order to support the idea that RTP treatment does not change membrane pore characteristics (as shown by Anant in the rhodamine diffusion study) I processed TEM samples that were not treated, RTPed in Ar, for 5 minutes at 800C, and RTPed in O2 at 800C for 5 minutes (I believe this was not a…
Following time lapse movies show the spreading of HUVECs on pncSi and glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~4 hrs. HUVEC spreading on pncSi HUVEC spreading on glass
Following images show the diffusion profile of rhodamine across pncSi as observed in the timelapse movies. Looking at the profiles no significant difference is observed between the non RTPed and RTPed samples. Non RTP sample: RTP sample:
I carried out another trial of spreading experiments and following time lapse movies show the spreading of fibroblasts on glass ( @ 37C, in L15 media supplemented with 10% FBS) over a time period of ~7 hrs. Fibroblast spreading on glass 1 Fibroblast spreading on glass 2 Time lapse movies for fibroblasts spreading on pncSi…
Spreading of cells (huvec and fibroblasts) on pncSi and glass was observed at ~37 C in L15 media (+ 10% FBS) for ~5 hours. Its observed that the cell spreading is pretty similar on both the substrates and the time lapse movies can be seen from the following links: huvec_glass_sprd huvec_membrane_sprd fibroblast_glass_sprd fibroblast_membrane_sprd Somehow its…
Diffusion of rhodamine was observed through pncSi samples from W620. This was done to compare the porosity of the membranes after RTP treatment. From the following movies (real time = 10 mins) it can be seen that there is no significant difference in diffusion between the samples after they are RTPed, as expected. Now, Henry…
Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours. I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture manuscript.
Couple of HUVEC adhesion trials were carried out and the results were found satisfactory as seen in the charts below.
HUVEC growth was monitored for 4 days on different substrates (glass, plastic and pncSi), this time in media (EGM) with and without serum. Growth in media w/o serum was observed to be much slower than the growth in media with FBS. Hence, media w/o serum can be used as a possible control for the growth…