Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities are due to the cells rolling of the edge of the raised pnc-Si substrate or…
HUVEC (P5) growth was monitored over 3 days on different substrates (glass coverslips, tissue culture treated & untreated plastic and RTPed pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi is higher followed by glass. As expected, the growth on treated plastic is…
A third fibroblast (L1 3T3, P22) growth test was conducted using glass, untreated and tissue culture treated plastic, and RTPed pnc-Si (W509) as substrates. Cells were grown in DMEM +10% FBS changed every 48 hours. R values are for the fit N=No*exp^(r*t), where r has units of divisions/hour. For this trial fibroblasts grown on glass,…
W502 and 504 were tested for burst pressure and air permeability. Only one sample from W502 was tested for burst pressure (the other broke while setting up and I wanted to conserve samples) with a burst pressure of 4.46 psi. The burst pressures for W504 ranged from 2.06 to 6.57 (n=3). Air permeability is shown…
HUVEC (P8) growth was monitored over 5 days on different substrates (glass coverslips, tissue culture treated plastic and pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi in two different trials were observed to be pretty much the same. Decrease in the growth…
A second fibroblast (L1 3T3, P21) growth test was conducted on RTP treated samples from W510, tissue culture plastic (polystyrene), and glass cover slips. Fibroblasts were cultured in DMEM with media changed every 48 hours. Cells were imaged using the live/dead assay. Fibroblasts divided every 29.9, 35, and 37.7 hours when grown on pnc-Si, glass,…
To improve our knowledge of pinhole formation I have started counting pinholes on W510, W512, and W513. I also am playing close attention to areas surrounding the samples that have been completely etched, to see if this correlates to pinhole number. A schematic of W510 with number of pinholes is shown below. I would like…
Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test. The results of the third growth experiment is shown below. After 86 hours the cells…
(Transferred from old blog) Wafer 303 samples, both non-RTP and RTP (800C for 5 minutes), were tested in cell media (DMEM supplemented with 10% FBS) or deionized water, and placed in the incubator (37C 5% CO2) or oven (approximately 37C). Discoloration, pH, and the bead assay were measured initially and every 24 hours. Deionized water…
(Transferred from old blog) Aim: Determine how quickly DMEM pH changes once removed from the incubator Methods and Materials: • non-sterile complete Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS • 6 and 24 well cell culture plates • 37 ºC 5% CO2 incubator 2 mL and 5 mL DMEM were placed in a…