HUVEC growth curve
HUVEC (P5) growth was monitored over 3 days on different substrates (glass coverslips, tissue culture treated & untreated plastic and RTPed pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi is higher followed by glass. As expected, the growth on treated plastic is faster than on the untreated plastic. The division rate of the cells was determined to be: 27.6, 46.1, 56.8 & 62.9 hours/division on pnc-Si, glass, treated & untreated plastic respectively.
What’s the consensus on the results over the last few weeks – is pnc-Si really a better substrate than these alternatives or is there something that is skewing the results? Can anything conclusive be said at this point? The posted data is pretty convincing to me, but I’d feel better if we could explain it. How can we determine why cells like pnc-Si?
Also, as I mentioned in my last post, we really need to start collecting this type of data only on the membranes. I assume that all this data is for cells both on the substrate and on the actual membranes themselves? What are the barriers that need to be overcome to run these true membrane experiments? It seems like we have plenty of material with various pinholes that is only really useful for cell culture work, I think.
Great work so far guys!!
Can we collect all our data for both cell types in the form of bar graphs. Plot per capita division rate as a function of surface and include error bars. HUVEC might appreciate the topology of the pnc-Si membranes.
I uploaded a Nalge Nunc reference on cell growth on difference surfaces to the Knowledge Wiki.
How are the attachment studies going?
From the results till now, it seems the pncSi is good for HUVECs in comparison to the fibroblasts. Its hard to say at this point about the reason behind this. This might be because of the topology or the access to the nutrients which might be different in case of pncSi in comparison to glass or tissue culture plastic.
Our next goal should be growing cells on the actual membrane. The cells were found happy on them too, but these weren’t done repeatedly. The main concern of growing the cells on membrane would be to keep them intact. Also, the normalization of the area on which the cells grow can be an issue while counting them.
Yes thats what it seems like. HUVECs are liking the pncSi more in comparison to fibroblasts.
Jim, the adhesion studies are going on. We are done with 4 trials and just looking for a way to compile them as the initial cell density of plating was different in each case. Henceforth, we would keep the density constant and go ahead with more trials. We could determine the cell density using the hemocytometer.