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Nanomembrane Research Group
  • Knowledge (Public)

    Nanoparticle Concentrations

    ByJim April 7, 2014February 26, 2019

    Knowledge of the particle number is critical in many of our applications. In trying to rationalize membrane ‘clogging’ by particles for example, it is wise to compare the particle density to pore density at the membrane. When conjugating protein to NPs, it is important to work in a molar excess of protein. In using nanoparticles…

    Read More Nanoparticle ConcentrationsContinue

  • NRG

    Surfactants tested to reduce agglomeration between IgG and 20 nm fluospheres

    ByJosh Winans March 30, 2014

    Mary Lee and I performed a quick test to determine the efficacy of different surfactants at reducing agglomeration between IgG and 20 nm fluospheres. Each solution is a combination of 50 uL 10 mg/mL IgG (in PBS) + 50 uL 20 nm red Invitrogen fluospheres + 500 uL of surfactant. We used 1 x PBS…

    Read More Surfactants tested to reduce agglomeration between IgG and 20 nm fluospheresContinue

  • NRG

    Endothelial Vacuolization Induced By Highly-permeable Silicon Membranes

    ByJim March 30, 2014

    Submitted to Acta Biomaterialia – March 15, 2014 Manuscript as PDF Check Status

    Read More Endothelial Vacuolization Induced By Highly-permeable Silicon MembranesContinue

  • Knowledge | Knowledge (Public)

    Protein Adsorption (are we saturating the substrate?)

    ByHenry March 25, 2014April 8, 2014

    Hi all, Today I would like to briefly mention the important parameters to consider when saturating a substrate with proteins by simple adsorption. More specifically, the concentration and time required to fully saturate a substrate ares estimated for systems with different geometry (see the PDF attachment): [NRG] surface adsoprtion The first-order approximations entailed in the above…

    Read More Protein Adsorption (are we saturating the substrate?)Continue

  • NRG

    NanoDays 3/29 & 3/30

    ByThomas Gaborski March 25, 2014March 25, 2014

    We still need help with NanoDays http://doodle.com/paq66krxtf7mt6tk There are two time slots: 10:45am-2pm 1:45-4pm Please wear your I Love Nano shirts from last year. I have a couple more size Medium and one size Small. The Rochester Museum & Science Center is on the corner of Goodman and East Ave.

    Read More NanoDays 3/29 & 3/30Continue

  • NRG

    XPS data on oxidized and surface treated NPN

    ByJosh Winans March 25, 2014

      Katia Lyubarskaya in Alex Shestopalov’s lab has been testing different surface conditions of oxidised NPN membranes.  I’ve placed her data below.  (We used chips from wafer 1070) We started by looking at chips that received different chemical/plasma/thermal processes to create an oxide layer.  The thermally treated sample has a significantly higher Oxygen peak, but…

    Read More XPS data on oxidized and surface treated NPNContinue

  • NRG

    Effects of Adenine Containing Diet on Urea Levels in Serum Drawn from Rats

    ByDean Johnson March 25, 2014

    In order to test the efficacy of any dialysis treatment, we need induce uremia in our animal model. We choose to forgo the surgical approach of performing a 5/6 nephrectomy via ligation for the simpler diet based approach. Feeding the animals a diet containing adenine is meant to raise the level of urea in the…

    Read More Effects of Adenine Containing Diet on Urea Levels in Serum Drawn from RatsContinue

  • NRG

    Shear Free Chemotaxis Manuscript (Submitted to LOC)

    ByJim March 25, 2014July 7, 2014

    Submitted on March 14, 2014 Submitted Manuscript (PDF) Check Status

    Read More Shear Free Chemotaxis Manuscript (Submitted to LOC)Continue

  • NRG

    Tecan Class Use

    ByJim March 24, 2014

    Dear McGrath Lab members, I would like to notify you that BME245 (Biomaterials Lab) students will be coming at following times (see below)  to use the plate reader. They will be accompanied with experienced TAs at all times. Wednesday- 3/26- 3:25 pm-9pm Thursday- 3/27- 9:30 am-12:25 pm and 3:25 pm- 6:05 pm. My apologies for…

    Read More Tecan Class UseContinue

  • NRG

    Synergistic Control of Mesenchymal Stem Cell Differentiation by Nanoscale Surface Geometry and Immobilized Growth Factors on TiO2 Nanotubes

    ByJosh Winans March 21, 2014

    von_der_Mark_2012 Here is some data on cell growth on different porosity TiO2 nanotubes.  The above paper is an earlier publication regarding the work presented in Poster form at PSST 2014.  Below are photos taken from the 4 quadrants of the poster.      

    Read More Synergistic Control of Mesenchymal Stem Cell Differentiation by Nanoscale Surface Geometry and Immobilized Growth Factors on TiO2 NanotubesContinue

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    • Home
    • Publications
    • Membranes
      • Common Chip Formats
      • Common Membranes
      • Microslit Membranes
    • Devices
      • µSiM
        • Geometry
        • µSiM CAD Files
        • Assembly
          • Protocols.io (µSiM Assembly)
          • Instructions
          • Common Issues and Troubleshooting Tips
        • Cell Culture Protocols
          • Top Well: hCMEC/D3
          • Top Well: HUVEC
          • Bottom Channel Culturing
          • Immunocytochemistry Protocol
          • Impact of Chip Orientation on Fluorescence Imaging
          • Permeability: In Situ Method
          • Permeability: Sampling Method
          • Cell Culture Common Issues and Troubleshooting Tips
      • SepCon®
        • Sepcon Assembly
        • Sepcon Video Protocol: Assembly
        • SepCon Gasket Silhouette File
        • SepCon Video Protocol: Wetting the membrane
        • SepCon Video Protocol: Disassembly
      • µSiM-DX
        • µSIM Video Protocol: Capture of Nanoparticles
    • Impact
      • TraCe-bMPS
      • HCIC
      • LOMP
      • SiMPore