Hi all, you might have read that we (Mike and i) have 100 % bonding efficiency in our latest PDMS-Si wafer bonding. On that same trial we have also successfully bonded PDMS to the new pnc-Si that Dave made for us (wafer 619, the rectilinear one with the V-shaped trenches leading to the Si-wafer well…
Hi All, Recently we have good results showing that we can bond PDMS to both glass and Si-wafer with UV Ozone treatment. We can even bond the PDMS to pnc-Si directly, although it is not clear whether the sealing is perfect. However, the success rate is not always 100% (either no bonding or partial bonding…
Last week I ran cell culture growth experiments on RTPed (800C 5 minutes in Ar) samples from W567 (single slit 100um x 1000um). For this study I tracked discoloration w/cells, membrane stability w/cells, and the bead assay w/cells to see if we can predict if a membrane used in cell culture is intact by monitoring…
Recently I tested track etched membranes and crystalline silicon (basically the backside of the membrane chips) following the sample buffer adsorption assay. This new data comprises the last two bars on the following chart. I’m surprised to see that crystalline silicon is so low in comparison to pnc-Si. This is something I’d like to test…
Time lapse movie of fibroblast spreading on the nanomembrane was captured over a period of ~ 8 hours. I was having some problem uploading the video here, thus have done so in the NRG knowledge page under the cell culture manuscript.
I’ve added a page on cell culture applications to the knowledge wiki. Watch this page and edit it if the topic is of interest to you. Remember the wikis provide a place to comment on the changes you make … and to compare and restore prior content … … so don’t be shy.
I recently received three pinhole-ridden wafers for cell-related experiments. All three have been RTPed at 800C for 5 minutes in argon, which is the typical procedure we have been using for cell culture. The bursts pressures for each wafer are shown below. W520 is weaker than 558 and 559; I plan on using the stronger…
Last week I mentioned that a two step anneal may help with the tears that are forming on the membranes when they are annealed post-etch. The recipe I used was a 10 C/s ramp to 600 C, hold 60 s, 10 C/s ramp to the setpoint, hold 60 s, ramp down. I annealed membranes with…
In order to test the sealing of the capillary tubing the PDMS I made a batch of PDMS with a recess in it (from a dime). Once this cured I used the UV Ozone process (@25C) to bond the PDMS to a glass microscope slide. When I arrived this morning the system was fully bound….
Maryna’s presentation