HUVEC adhesion update
Couple of HUVEC adhesion trials were carried out and the results were found satisfactory as seen in the charts below.
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Membrane Anneal Study: W510X, W600 Testing
I recently tested W510X and W600 described by Dave here for air permeability, rhodamine diffusion, and burst pressure. No air permeability was observed; even a sample with one very small pinhole (W600 800C anneal) only had a permeability of 11 cc/atm*cm2*s. To verify the lack of pores I performed rhodamine diffusion on the W600 700C…
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HUVEC growth kinetics with serum free media as control
HUVEC growth was monitored for 4 days on different substrates (glass, plastic and pncSi), this time in media (EGM) with and without serum. Growth in media w/o serum was observed to be much slower than the growth in media with FBS. Hence, media w/o serum can be used as a possible control for the growth…
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PDMS-Membrane Bonding Update
This is just an update on the progress being made towards creating a closed flow cell by bonding PDMS to our membrane chips. For those who were not at the meeting on Tuesday we have successfully bonded PDMS to both glass cover slips as well as out membrane chips using the UV Ozone cleaner. Yesterday…
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DI water buffer or thiol passivation?
I hope to bring this question to our group meeting. As we discussed before, we know that Cl- can be absorbed at the gold surface to screen the applied positive charge. We are using R6G as the filtration species, which is positively charged. So we must either use DI water as the buffer solution (we…
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Desalting: Broken and Pinholed Membranes
Desalting of 4M NaCl in deionized water was performed using our membranes in the SepCon format. I ran the SepCons in the same as way as the previous study, using a low stir rate and floating the SepCons. Once again, a conductivity probe was used to measure the amount of salt in the dialysate. Transport…
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Membrane anneal study
Last week, we reported that it was possible to anneal membranes after they had been etched. Below is a summary of results from membranes that were annealed over a range of temps and under different conditions. w600 (20 nm oxide) w510X (40 nm oxide) amorph. anneal 700 C no defects no defects amorph. anneal 750…
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new chamber with brief description
As per Chris request, here is a photo with a brief description. Sorry the image is still not good… the surface of the plastic reflects lights and it is transparent, plus I am using a poor camera. But I hope the description is useful and anyway tomorrow we can check it out in the meeting!
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new version of the diffusion chamber
Since Paul sent an email saying he will be on leave from this week, I spent some time in the hopeman metal workshop with Paul working on our second diffusion chamber before he leaves. We actually failed four substrates before we made it. Here is the enhanced version. First, we made the hole through the…
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diffusion observed using the prototype chamber with background noise
Last week Jess and I did some diffusion tests using the prototype chamber. We observed there was some fluorescence background noise from the plastic substrate. So we decided to make the buffer well (the hole on the substrate) all the way through the Polycarbonate sheet and seal the well with a slide. We can see…