Previously we have worried about the validity of our adsorption data considering we have used it to test both sides of anisotropic membranes that have cutoffs below the size of albumin. Today we came up with the idea that albumin can be used as a probe for adsorption, not as simply a model protein in…
HUVEC adhesion was measured on glass, tissue culture plastic, and pnc-Si, with poly-lysine coated plastic and teflon coverslips as positive and negative controls. Adhesion on pnc-Si was low, this could be due to cells being lost through broken slits or possibly some other unknown factor. Adhesion tests are continuing, with the use of solid Si…
Finally on Friday all the pieces came in that we ordered to connect the peristaltic pump tubing to the 238umOD capillary tubing. This is our first order, after last weeks meeting we ordered a smaller OD capillary tubing (164um) with the same 100umID. I connected all the pieces and attached the micropump to make sure…
UPDATE: Teflon data now include Original Post: Refer back to Part I, and Part II This weekend I ran a bunch of gels to flesh out the adsorption data a bit more. I tested MicroCons, NanoSeps, and teflon and reran the initial Pnc-Si gel to get rid of negative results due to uneven staining. To…
Inspired by JP, we decided to try to etch amorphous (unannealed) wafers this week to see if pinhole density changed. To my surprise, the amorphous membranes had no pinholes! Furthermore, I tried to anneal the membranes after the etch and they went flat indicating crystallization! Refer to the table below for the results. Wafer 565:…
Over the last few days I have spent some time learning the basics of the COMSOL modeling software. I have just started to understand the basics and I just wanted to show an example of what this program is capable of. Below are images of flow modeled through a “non-uniform pore”. This model assumes water…
Fibroblast adhesion experiment was done using poly-lysine coated plastic and teflon coverslips as positive and negative controls repectively. Poly-lysine is a polypeptide that gives the cells a sticky surface to adhere to. Teflon coverslips are used as negative control because they do not promote cell adhesion. Difference in morphology of cells could also be seen…
I worked with Paul this afternoon and finally we highly suspect the raw material used for the prototype diffusion chamber could be Polycarbonate. Paul could not provide direct evidence for the material source since he did not keep any purchase orders or materials data sheet… However, we did check our chamber with another plastic board…
Attached are the photos taken under DIC and Rhodamine channels, using the new diffusion chamber. I can clearly observe Rhodamine pass to the lower hole of the chamber, under the Rhodamine channel by eye ball. But from what I got using the Matlab software, it looks weird because both DIC and Rhodamine photos look very…
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