I ran a few tests with rhodamine to see if the fluorescence will transfer across the cell. The problem I keep having is that the stir cell set-up is very sensitive. If the wing nuts are tightened too much the membrane will break. But if they are too loose the liquids will leak out. Luckily…
The controls of the micropump are in units of frequency, voltage, and wave form, not in flow rates. Based on the manufacturers website they claim that the flow rate has a linear relation with voltage. I took some time to create calibration curves at two fixed frequencies (50Hz and 150Hz). I simply allowed the system…
Just wanted to let everyone know that the fused silica capillary tubing and adapter needed to connect them to the pump tubing have been ordered and should be in hopefully by Friday (Monday at the latest).
Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is only the prototype, there must be upside space for improvement.
We have been wondering about the reason for discoloration for a while. This could be due to the pH of the solution or the concentration of the salts in the solution. To narrow down our vision on this, first we tried using solutions with different pH values ranging from 4 to 9 in room temperature….
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are not on the slits but rather on substrate surrounding. It seems that HUVEC proliferate more…
Henry and I just got back from our meeting with Dean over at RIT to talk with him about the production process for making our proposed flow dialysis system. While we were there Dean described in detail the entire production process including the anodic bonding and the parylene deposition. We also returned with membrane samples…
Mike and I tested the movement of Rhodamine between the two cell channels to prove that the Rhodamine particles do diffuse through the membrane. After inspection in the microscope we saw that the fluorescence from the Rhodamine does transfer from one channel to the other across the membrane. However, we also noticed that the Rhodamine…
Adhesions tests are being performed to see if cellular attachment varies between our material, glass, and plastic. These experiments are motivated by lower initial cell densities observed on pnc-Si in growth tests; we are investigating if the lower initial densities are due to the cells rolling of the edge of the raised pnc-Si substrate or…
HUVEC (P5) growth was monitored over 3 days on different substrates (glass coverslips, tissue culture treated & untreated plastic and RTPed pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi is higher followed by glass. As expected, the growth on treated plastic is…