Electron Beam Evaporator Protocol (V_1.0) – Lingyun Here is the first version protocol of the electron beam evaporator at RIT clean room. There is not too much to put down here – the best way to learn this is to attend the training facing the real machine tool. I will try to provide some photos…
A third fibroblast (L1 3T3, P22) growth test was conducted using glass, untreated and tissue culture treated plastic, and RTPed pnc-Si (W509) as substrates. Cells were grown in DMEM +10% FBS changed every 48 hours. R values are for the fit N=No*exp^(r*t), where r has units of divisions/hour. For this trial fibroblasts grown on glass,…
Wafer#502 was coated with 10nm_Ti & 15nm_Au on Friday. I will pick out pinhole free membranes later today. I will also try to get TEM images at Karen’s lab (medical center). // The RIT clean room is closed this week.
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done. This data has all been obtained using the more vigorous washing step. All densitometry performed…
In our previous adsorption study, all of the surfaces seemed to adsorb a similar amount of protein. We thought this might be due to ineffective washing. In this study I tried a better washing technique: Incubate polymer membranes, pnc-Si chips, and glass in 1mg/mL BSA for 30min Rinse by vigorously swishing in 1500mL PBS for…
Glass chips and pnc-Si membranes were incubated in 1mg/mL FITC labeled BSA for 30min. They were vigorously washed in a large beaker of PBS. Chips were placed on a slide and imaged with fluorescence scope. For this study, it seems that the fluid surrounding the chip was much brighter than the chip itself. The following…
Wafers 525 & 527 have a 1000 Å dry thermal oxide mask with all three films on the frontside sputtered. Both these wafers had a high density of pinholes. The pores are also irregular in shape and seem to have material “hanging” off the edges of the pores. Could this be a consequence of depositing…
W502 and 504 were tested for burst pressure and air permeability. Only one sample from W502 was tested for burst pressure (the other broke while setting up and I wanted to conserve samples) with a burst pressure of 4.46 psi. The burst pressures for W504 ranged from 2.06 to 6.57 (n=3). Air permeability is shown…
HUVEC (P8) growth was monitored over 5 days on different substrates (glass coverslips, tissue culture treated plastic and pnc-Si from W510). The growth curve is as shown below and it can be observed that the growth rate on pncSi in two different trials were observed to be pretty much the same. Decrease in the growth…
A second fibroblast (L1 3T3, P21) growth test was conducted on RTP treated samples from W510, tissue culture plastic (polystyrene), and glass cover slips. Fibroblasts were cultured in DMEM with media changed every 48 hours. Cells were imaged using the live/dead assay. Fibroblasts divided every 29.9, 35, and 37.7 hours when grown on pnc-Si, glass,…