Last week I attempted another HUVEC growth study on Si, coverslips (glass), and culture wells (plastic). The cells were seeded at too low of a density on the membrane so I adjusted my protocol and started a third growth test. The results of the third growth experiment is shown below. After 86 hours the cells…
Here are two new designs for the new diffusion cells. Design_2 could be a better solution as it separates the two connections and uses screws to make the wire contacts to outside circuit. Please feed back any ideas to improve these designs. Dave and I probably will start working on it some time this week….
As many of you know, we have been having a lot of trouble with the new etch cell and have not been able to produce high quality membranes. Yesterday, we discovered that the stainless steel used to fabricate the new cell is different than that of the original cell. Here are the EDAX spectra: Old…
In anticipation of the pnc-Si separation paper, I have performed a couple of preliminary separations using NanoSeps and MicroCons. I applied 200ul of 1:20 brain extract ( ~.9mg/mL) to 100k NanoSeps and MicroCons, and I spun the samples for 3 minutes at 14000g. I had wanted to collect the retentate as is from the top,…
Previously we have studied protein adsorption to pnc-Si membranes by TEM and fluorescence. Neither of these methods will work with polymer membranes. Polymer membranes are too thick to view using TEM. Polymer membranes also autofluoresce creating a lot of noise while using our fluorescence method. Here I outline a new method of studying adsorption to…
(Transferred from old blog) From the above discoloration array it can be inferred that the RTP treated samples in the incubator are discoloring in much slower rate than the ones on the benchtop. Thus, incubator conditions prevent the chips from discoloring. The reason behind this could be the carbon dioxide present in th incubator which…
(Transferred from old blog) The above TEM images show that RTP (800C, 5 min) treated samples (from W334) have their pores intact in salt over a period of time! 1M and 2M NaCl solutions were used and TEM samples were taken at different time points. There is some deformation observed near the edges of the…
(Transferred from old blog) Wafer 303 samples, both non-RTP and RTP (800C for 5 minutes), were tested in cell media (DMEM supplemented with 10% FBS) or deionized water, and placed in the incubator (37C 5% CO2) or oven (approximately 37C). Discoloration, pH, and the bead assay were measured initially and every 24 hours. Deionized water…
(Transferred from old blog) Aim: Determine how quickly DMEM pH changes once removed from the incubator Methods and Materials: • non-sterile complete Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FBS • 6 and 24 well cell culture plates • 37 ºC 5% CO2 incubator 2 mL and 5 mL DMEM were placed in a…
It took me a little while to figure out how to turn the .avi file into a .gif, but here is an image of the diffusion through 501. Due to a problem with MatLab, it took about 5min before I started taking images of the diffusion. So this starts at ~5 min after the 100mM…