New metal coated wafer#502

As an introduction, my name is Alec Salminen. I received my BS in Biomedical Engineering at the University of Arkansas and am currently in my first year of the BME PhD program at the University of Rochester. As my rotation in the McGrath lab gets underway, I would like to share with the group my…

This is just a little update on the results of the pnc-Si, cellulose, and pes separations.  When I added up the retentate and filtrate, it didn’t always equal the intensity of the starting sample (keeping in mind that I was unable to collect filtrate from PES and should re-try that separation). I took the percent…

A couple weeks ago, I presented a potential figure for my paper that didn’t make sense.  So, I did a couple more controls and put together this new figure.  This is the “diffusion through ‘non-porous’ SC348/cut-off of nonporous membranes” figure.  I redid it to include SC348 (the nonporous sample) and ‘typical’ pnc-Si as % of…

One of the ongoing efforts with endothelial cell culture on pnc-Si is to confirm that the holes in these cells are actually vacuoles.  In my last post, I tried to do this with FITC-Dextran and TMRIA with indefinite results.  I ordered some 5-6-carboxytetramethylrhodamine (TAMRA, C-300 from Invitrogen) to do this test again.  This rhodamine dye…

About a month ago, I posted some preliminary data that showed NaCl diffusion through SC348.  This was motivated by my observation of vacuole formation on SC348.  Since my hypothesis is that cell culture substrate permeability is the key factor for vacuole formation, cells shouldn’t have formed vacuoles on SC348.  Unless it is actually porous.  This…

Last week Jess helped me set up the diffusion cell with human albumin and cytochrome c. We ran 3 cells and used membranes from wafer 565. The membranes used were -1,4 (retentate 1), 3,3 (retentate 2), and 4,2 (retentate 3). The gel is posted below: The columns are (from left): Protein, control, retentate 1, filtrate…